| Literature DB >> 22430125 |
Boh-Ram Kim1, Hye-Jin Shin, Joo-Young Kim, Hyun-Jung Byun, Jeong Heon Lee, Young Kwan Sung, Seung Bae Rho.
Abstract
Recently, we found that carbonic anhydrase IX (CA9) modulates tumor-associated cell migration and invasion, and then identified dickkopf-1 (DKK-1) as a novel CA9-interacting protein. In this study, we have determined the binding regions that are required for interaction between CA9 and DKK-1 through in vitro and in vivo. The N-terminal domain of CA9 is participated to interact with the Val(60)-Tyr(168) site of DKK-1. We also observed that DKK-1 inhibits endothelial cell angiogenesis of CA9 in tumorigenesis. Furthermore, induction of CA9-mediated mTOR phosphorylation and angiogenesis was significantly inhibited by over-expression of DKK-1. Taken together, these findings identify DKK-1 as a potential factor in the regulation of CA9 cellular homeostasis and also suggest a new possible role for DKK1-1 in tumorigenesis.Entities:
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Year: 2012 PMID： 22430125 DOI： 10.1016/j.cellsig.2012.03.002
Source DB: PubMed Journal: Cell Signal ISSN： 0898-6568 Impact factor: 4.315