UNLABELLED: Oxidative stress has been associated with diabetic complications like nephropathies. Recent studies indicate that ursodeoxycholic acid (UDCA) may be beneficial preventing diabetes-induced oxidative stress and secondary complications. Thus, we study if the UDCA-treatment decreases the expression of sodium-glucose cotransporter (SGLT2) and the oxidative stress in the kidney of diabetic rats. METHODS: The diabetes model was established by intraperitoneal injection of streptozotocin (50mg/kg). SGLT2 expression was evaluated by western blot and RT-PCR. Oxidative stress was assessed by catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase activities (SOD) and immunohistochemical analysis of 3-nitrotyrosine (3-NT). RESULTS: Streptozotocin-induced diabetes caused hyperglycemia and lower body weight. The SGLT2 expression and mRNA levels increased in cortex of kidney from diabetic rats. The CAT activity decreased in cortex and medulla from diabetic rats, otherwise the GPx activity increased. Furthermore the 3-NT staining of kidney from diabetic rats increased compared to control rats. The UDCA treatment was able to decrease hyperglycemia and prevents the SGLT2 over-expression, restores the CAT and GPX activities and decreases 3-NT staining. CONCLUSION: The UDCA treatment prevents the over-expression of SGLT2 and oxidative stress in kidney of diabetic rats.
UNLABELLED: Oxidative stress has been associated with diabetic complications like nephropathies. Recent studies indicate that ursodeoxycholic acid (UDCA) may be beneficial preventing diabetes-induced oxidative stress and secondary complications. Thus, we study if the UDCA-treatment decreases the expression of sodium-glucose cotransporter (SGLT2) and the oxidative stress in the kidney of diabeticrats. METHODS: The diabetes model was established by intraperitoneal injection of streptozotocin (50mg/kg). SGLT2 expression was evaluated by western blot and RT-PCR. Oxidative stress was assessed by catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase activities (SOD) and immunohistochemical analysis of 3-nitrotyrosine (3-NT). RESULTS:Streptozotocin-induced diabetes caused hyperglycemia and lower body weight. The SGLT2 expression and mRNA levels increased in cortex of kidney from diabeticrats. The CAT activity decreased in cortex and medulla from diabeticrats, otherwise the GPx activity increased. Furthermore the 3-NT staining of kidney from diabeticrats increased compared to control rats. The UDCA treatment was able to decrease hyperglycemia and prevents the SGLT2 over-expression, restores the CAT and GPX activities and decreases 3-NT staining. CONCLUSION: The UDCA treatment prevents the over-expression of SGLT2 and oxidative stress in kidney of diabeticrats.
Authors: Sandra Feijóo-Bandín; Alana Aragón-Herrera; Manuel Otero-Santiago; Laura Anido-Varela; Sandra Moraña-Fernández; Estefanía Tarazón; Esther Roselló-Lletí; Manuel Portolés; Oreste Gualillo; José Ramón González-Juanatey; Francisca Lago Journal: Int J Mol Sci Date: 2022-05-18 Impact factor: 6.208
Authors: José Pedraza-Chaverri; Laura G Sánchez-Lozada; Horacio Osorio-Alonso; Edilia Tapia; Alexandra Scholze Journal: Oxid Med Cell Longev Date: 2016-06-27 Impact factor: 6.543
Authors: Ele Ferrannini; Ricardo Pereira-Moreira; Marta Seghieri; Eleni Rebelos; Aglécio L Souza; Valeria B Chueire; Caterina Arvia; Elza Muscelli Journal: BMJ Open Diabetes Res Care Date: 2020-05