| Literature DB >> 22427496 |
Andreas Kaczmarczyk1, Julia A Vorholt, Anne Francez-Charlot.
Abstract
Here, we suggest that natural streptomycin resistance of many sphingomonads resides within rpsL. We constructed a dominant, streptomycin-sensitive rpsL allele and demonstrated its use as a counterselection marker in several sphingomonads. An rpsL-based markerless gene deletion system was developed and validated by deleting four genes in Sphingomonas sp. strain Fr1.Entities:
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Year: 2012 PMID: 22427496 PMCID: PMC3346355 DOI: 10.1128/AEM.07347-11
Source DB: PubMed Journal: Appl Environ Microbiol ISSN: 0099-2240 Impact factor: 4.792