| Literature DB >> 22425785 |
Catherine Baugé1, Nicolas Girard, Sylvain Leclercq, Philippe Galéra, Karim Boumédiene.
Abstract
Interleukin-1β (IL-1β), a key-cytokine in osteoarthritis, impairs TGFβ signaling through TβRII down-regulation by increasing its degradation. Here, we investigated the molecular mechanism that controls TßRII fate in IL-1ß treated cells. Chondrocytes were treated with IL-1ß in the presence of different inhibitors. TßRII and Cav-1 expression were assayed by Western blot and RT-PCR. We showed that IL-1ß-induced degradation of TßRII is dependent on proteasome and on its internalization in caveolae. In addition, IL-1ß enhances Cav-1 expression, a major constituent of lipid raft. In conclusion, we enlighten a new mechanism by which IL-1ß antagonizes TGFß pathway and propose a model of TßRII turnover regulation upon IL-1ß treatment.Entities:
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Year: 2012 PMID： 22425785 DOI： 10.1016/j.bbamcr.2012.02.017
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002