OBJECTIVES: The purpose of this study was to assess the prevalence of HSV-1 and HSV-2 in sexually active women who participated in the cervical cancer screening program in Natal, Brazil. STUDY DESIGN: The study included 261 sexually active women resident in the metropolitan area of Natal, Brazil and attending a public clinic for cervical screening. From each participant, a sample of exfoliated uterine cervical cells was collected, using a cytobrush which was conditioned in a tube containing a preserving solution (PBS+vancomycin+nystatin) and sent to a laboratory where it was processed for DNA extraction. The samples were analyzed for the presence of HSV-1 and HSV-2 DNA in separate reactions by PCRs using specific primers. RESULTS: HSV-1 in genital infection is four times more prevalent than HSV-2 in the population analyzed. The highest prevalence rates for both viruses were found in women aged 31-39years. We did not observe any association between the presence of both virus serotypes and socio-demographic characteristics in the population studied, nor with some classical risk factors for sexually transmitted diseases. CONCLUSIONS: HSV-1 was the major cause of genital infection by Herpes simplex virus in the women included in this study. No association was found between HSV infection and the socio-demographic characteristics or some classical risk factors for sexually transmitted diseases.
OBJECTIVES: The purpose of this study was to assess the prevalence of HSV-1 and HSV-2 in sexually active women who participated in the cervical cancer screening program in Natal, Brazil. STUDY DESIGN: The study included 261 sexually active women resident in the metropolitan area of Natal, Brazil and attending a public clinic for cervical screening. From each participant, a sample of exfoliated uterine cervical cells was collected, using a cytobrush which was conditioned in a tube containing a preserving solution (PBS+vancomycin+nystatin) and sent to a laboratory where it was processed for DNA extraction. The samples were analyzed for the presence of HSV-1 and HSV-2 DNA in separate reactions by PCRs using specific primers. RESULTS:HSV-1 in genital infection is four times more prevalent than HSV-2 in the population analyzed. The highest prevalence rates for both viruses were found in women aged 31-39years. We did not observe any association between the presence of both virus serotypes and socio-demographic characteristics in the population studied, nor with some classical risk factors for sexually transmitted diseases. CONCLUSIONS:HSV-1 was the major cause of genital infection by Herpes simplex virus in the women included in this study. No association was found between HSV infection and the socio-demographic characteristics or some classical risk factors for sexually transmitted diseases.
Authors: Paula A Suazo; Francisco J Ibañez; Angello R Retamal-Díaz; Marysol V Paz-Fiblas; Susan M Bueno; Alexis M Kalergis; Pablo A González Journal: Mediators Inflamm Date: 2015-03-30 Impact factor: 4.711
Authors: Cleine Aglacy Nunes Miranda; Erika Galvão Lima; Diego Breno Soares de Lima; Ricardo Ney Oliveira Cobucci; Maria da Conceição de Mesquita Cornetta; Thales Allyrio Araújo de Medeiros Fernandes; Paulo Roberto Medeiros de Azevedo; Jenner Chrystian Veríssimo de Azevedo; Josélio Maria Galvão de Araújo; José Veríssimo Fernandes Journal: ISRN Obstet Gynecol Date: 2014-03-11
Authors: Arun V Iyer; Bapi Pahar; Vladimir N Chouljenko; Jason D Walker; Brent Stanfield; Konstantin G Kousoulas Journal: Virol J Date: 2013-10-28 Impact factor: 4.099