Literature DB >> 22422854

The small membrane filter method of microscopy to diagnose pulmonary tuberculosis.

Kevin P Fennelly1, Carlos Gustavo Morais, David Jamil Hadad, Solange Vinhas, Reynaldo Dietze, Moises Palaci.   

Abstract

We modified microscopy for acid-fast bacilli to diagnose tuberculosis (TB) using small membrane filters (SMFs) after special processing and prefiltration. With the first specimen obtained from each of 335 persons suspected of having TB, the sensitivity of the new SMF method using fluorescence microscopy (FM) was 89% (95% confidence interval [CI]: 80%, 95%). This was significantly better (P = 0.0001) than the sensitivity of routine FM of centrifuged specimens of 60% (95% CI: 49%, 71%) or that of direct sputum smears of 56% (95% CI: 40%, 72%).

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Year:  2012        PMID： 22422854      PMCID： PMC3372110          DOI： 10.1128/JCM.00572-12

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  6 in total

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2.  Evaluation of processing methods to equitably aliquot sputa for mycobacterial testing.

Authors:  David Jamil Hadad; Carlos Gustavo Vieira Morais; Solange A Vinhas; Kevin P Fennelly; Reynaldo Dietze; Cristina Paula Nascimento; Moises Palaci
Journal:  J Clin Microbiol       Date:  2012-01-25       Impact factor: 5.948

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4.  Acid-fast microscopy on polycarbonate membrane filter sputum sediments.

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Review 6.  Laboratory-associated infections and biosafety.

Authors:  D L Sewell
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  6 in total
  7 in total

1.  Evaluation of a modified small membrane filtration method.

Authors:  Kevin P Fennelly
Journal:  J Clin Microbiol       Date:  2014-12       Impact factor: 5.948

2.  Increased sensitivity in diagnosis of tuberculosis in HIV-positive patients through the small-membrane-filter method of microscopy.

Authors:  Patrícia Quincó; Samira Bührer-Sékula; Walber Brandão; Rossiclea Monte; Silvia Leopoldina Souza; Valeria Saraceni; Moises Palaci; Reynaldo Dietze; Marcelo Cordeiro-Santos
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Journal:  J Clin Microbiol       Date:  2014-05-07       Impact factor: 5.948

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Journal:  J Clin Microbiol       Date:  2016-03-30       Impact factor: 5.948

5.  Detection and Quantification of Mycobacterium tuberculosis in the Sputum of Culture-Negative HIV-infected Pulmonary Tuberculosis Suspects: A Proof-of-Concept Study.

Authors:  Guillermo Madico; Moses Mpeirwe; Laura White; Solange Vinhas; Beverley Orr; Patrick Orikiriza; Nancy S Miller; Mary Gaeddert; Juliet Mwanga-Amumpaire; Moises Palaci; Barry Kreiswirth; Joe Straight; Reynaldo Dietze; Yap Boum; Edward C Jones-López
Journal:  PLoS One       Date:  2016-07-08       Impact factor: 3.240

6.  Development and evaluation of novel bio-safe filter paper-based kits for sputum microscopy and transport to directly detect Mycobacterium tuberculosis and associated drug resistance.

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7.  It is health that is real wealth & not pieces of gold & silver.

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  7 in total

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