Literature DB >> 22422025

An efficient and simple method for site-selective modification of O6-methyl-2'-deoxyguanosine in DNA.

Kazumitsu Onizuka1, Takamasa Nishioka, Zhichun Li, Daichi Jitsuzaki, Yosuke Taniguchi, Shigeki Sasaki.   

Abstract

O(6)-Methyl-2'-deoxyguanosine (O(6)-Me-dG) is a mutagenic nucleotide in DNA. O(6)-Me-dG in DNA was rapidly and selectively modified by a functionality transfer reaction using the ODN incorporating 6-S-functionalized thioguanosine. Subsequent labelling of O(6)-Me-dG with the fluorescent FAM or biotin group via click chemistry has permitted the sensitive and selective detection of O(6)-Me-dG in DNA.

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Year:  2012        PMID： 22422025     DOI： 10.1039/c2cc17621a

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  4 in total

1.  Recognition of O6-benzyl-2'-deoxyguanosine by a perimidinone-derived synthetic nucleoside: a DNA interstrand stacking interaction.

Authors:  Ewa A Kowal; Rahul R Lad; Pradeep S Pallan; Elizabeth Dhummakupt; Zdzislaw Wawrzak; Martin Egli; Shana J Sturla; Michael P Stone
Journal:  Nucleic Acids Res       Date:  2013-06-08       Impact factor: 16.971

2.  Remarkable acceleration of a DNA/RNA inter-strand functionality transfer reaction to modify a cytosine residue: the proximity effect via complexation with a metal cation.

Authors:  Daichi Jitsuzaki; Kazumitsu Onizuka; Atsushi Nishimoto; Ikuya Oshiro; Yosuke Taniguchi; Shigeki Sasaki
Journal:  Nucleic Acids Res       Date:  2014-06-23       Impact factor: 16.971

Review 3.  Labeling and sequencing nucleic acid modifications using bio-orthogonal tools.

Authors:  Hui Liu; Yafen Wang; Xiang Zhou
Journal:  RSC Chem Biol       Date:  2022-06-22

4.  In-Gene Quantification of O(6)-Methylguanine with Elongated Nucleoside Analogues on Gold Nanoprobes.

Authors:  Ioannis A Trantakis; Arman Nilforoushan; Heidi A Dahlmann; Celine K Stäuble; Shana J Sturla
Journal:  J Am Chem Soc       Date:  2016-07-01       Impact factor: 15.419
  4 in total

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