OBJECTIVE: Diabetes causes changes in the salivary glands and in the composition of saliva, as well as symptoms such as dry mouth and hyposalivation. Therefore, this study aimed at investigating changes in salivary secretion and composition, in response to parasympathetic stimuli, in diabetic rats induced with streptozotocin. DESIGN: Diabetes was induced by a single intraperitoneal injection of streptozotocin. Thirty days after diabetes induction, the animals were anaesthetized and salivation was stimulated by an intraperitoneal injection of Pilocarpine (0.6mg/kg body weight) dissolved in distilled water. Saliva was collected for 40min and immediately stored at -80°C until analysis. The salivary flow rate, amount of total protein, amylase and peroxidase activities, and free and total sialic acid contents were measured. RESULTS: Salivary flow rate was reduced in the diabetic group (p<0.05). Moreover, increases in total protein amount and in amylase and peroxidase activities were observed in diabetic animals. No difference was observed for free sialic acid content between groups. On the other hand, a significantly decrease in the total sialic acid content was observed in the diabetic group (p<0.05). CONCLUSIONS: Our findings suggest that a decrease in sialic acid in the saliva of diabetic animals can be related to xerostomia reported by diabetic patients. However, further clinical trials are needed to verify if the decrease in sialic acid also occurs in human saliva.
OBJECTIVE:Diabetes causes changes in the salivary glands and in the composition of saliva, as well as symptoms such as dry mouth and hyposalivation. Therefore, this study aimed at investigating changes in salivary secretion and composition, in response to parasympathetic stimuli, in diabeticrats induced with streptozotocin. DESIGN:Diabetes was induced by a single intraperitoneal injection of streptozotocin. Thirty days after diabetes induction, the animals were anaesthetized and salivation was stimulated by an intraperitoneal injection of Pilocarpine (0.6mg/kg body weight) dissolved in distilled water. Saliva was collected for 40min and immediately stored at -80°C until analysis. The salivary flow rate, amount of total protein, amylase and peroxidase activities, and free and total sialic acid contents were measured. RESULTS: Salivary flow rate was reduced in the diabetic group (p<0.05). Moreover, increases in total protein amount and in amylase and peroxidase activities were observed in diabetic animals. No difference was observed for free sialic acid content between groups. On the other hand, a significantly decrease in the total sialic acid content was observed in the diabetic group (p<0.05). CONCLUSIONS: Our findings suggest that a decrease in sialic acid in the saliva of diabetic animals can be related to xerostomia reported by diabeticpatients. However, further clinical trials are needed to verify if the decrease in sialic acid also occurs in human saliva.
Authors: Ana Carolina Romero; Cassia Toledo Bergamaschi; Douglas Nesadal de Souza; Fernando Neves Nogueira Journal: PLoS One Date: 2016-02-09 Impact factor: 3.240
Authors: M Knaś; M Maciejczyk; I Daniszewska; A Klimiuk; J Matczuk; U Kołodziej; D Waszkiel; J R Ładny; M Żendzian-Piotrowska; A Zalewska Journal: J Diabetes Res Date: 2016-07-13 Impact factor: 4.011
Authors: Ann-Marie Torregrossa; Larissa Nikonova; Michelle B Bales; Maria Villalobos Leal; James C Smith; Robert J Contreras; Lisa A Eckel Journal: PLoS One Date: 2014-08-27 Impact factor: 3.240
Authors: Piotr Żukowski; Mateusz Maciejczyk; Jan Matczuk; Krzysztof Kurek; Danuta Waszkiel; Małgorzata Żendzian-Piotrowska; Anna Zalewska Journal: Oxid Med Cell Longev Date: 2018-01-30 Impact factor: 6.543