Identification and functional analysis of novel micro-RNAs in rat dorsal root ganglia after sciatic nerve resection.

Peripheral nerve injures are quite common in clinical practice, and many studies have tried to explore the underlying molecular mechanisms. This study focuses on the identification and functional analysis of novel miRNAs in rat dorsal root ganglia (DRGs) following sciatic nerve resection, which is a classic model for studying peripheral nerve injury and regeneration. By using Solexa sequencing, computational analysis, Q-PCR verification, and Dicer knockdown assay, 114 novel miRNAs in rats were identified, of which 51 novel miRNAs were first reported in rat DRGs, and 63 novel miRNAs were produced at days 1, 4, 7, and 14 following sciatic nerve resection. We further predicted target genes of these miRNAs and analyzed the biological processes in which they were involved. The identified biological processes were consistent with the time frame of peripheral nerve injury and regeneration, revealing that these miRNAs were genuine miRNAs related to nerve regeneration. Our data provide an important resource for the future study of function and regulation of these miRNAs and contribute to elucidation of tyhe molecular mechanisms responsible for peripheral nerve injury and regeneration.