BACKGROUND: Sorghum possesses phenolic compounds that are health-promoting constituents of the grain. There are approximately 40 000 sorghum accessions, many of which have not been evaluated for the grain's health-promoting potential. Conventional methods for measuring total phenolic content, flavonoid content and 2,2-diphenyl-1-picrylhydrazyl (DPPH)-scavenging capacity are time-consuming and labour-intensive, resulting in low overall throughput. The objective of this study was to develop a high-throughput screening assay for large sorghum sample sets to determine flavonoid and phenolic content and to modify existing DPPH and total phenolic assays. RESULTS: The 96-well assays exhibited a correlation of > 0.9 with the conventional assays. The 96-well assays allowed for up to 64 samples to be run per day compared with 20-24 samples (depending on the test) for the conventional methods. The 96-well assays had excellent accuracy (97.65-106.16% recovery), precision (1.06-8.28% coefficient of variation (CV)) and reproducibility (1.32-2.13% CV inter-day and 1.36-2.09% CV intra-day). CONCLUSION: The high-throughput 96-well plate method proved to be as robust and reproducible as the conventional method for determining total phenolic content, flavonoid content and DPPH-scavenging capacity in either sorghum bran or flour. Published 2012 by John Wiley & Sons, Ltd.
BACKGROUND:Sorghum possesses phenolic compounds that are health-promoting constituents of the grain. There are approximately 40 000 sorghum accessions, many of which have not been evaluated for the grain's health-promoting potential. Conventional methods for measuring total phenolic content, flavonoid content and 2,2-diphenyl-1-picrylhydrazyl (DPPH)-scavenging capacity are time-consuming and labour-intensive, resulting in low overall throughput. The objective of this study was to develop a high-throughput screening assay for large sorghum sample sets to determine flavonoid and phenolic content and to modify existing DPPH and total phenolic assays. RESULTS: The 96-well assays exhibited a correlation of > 0.9 with the conventional assays. The 96-well assays allowed for up to 64 samples to be run per day compared with 20-24 samples (depending on the test) for the conventional methods. The 96-well assays had excellent accuracy (97.65-106.16% recovery), precision (1.06-8.28% coefficient of variation (CV)) and reproducibility (1.32-2.13% CV inter-day and 1.36-2.09% CV intra-day). CONCLUSION: The high-throughput 96-well plate method proved to be as robust and reproducible as the conventional method for determining total phenolic content, flavonoid content and DPPH-scavenging capacity in either sorghum bran or flour. Published 2012 by John Wiley & Sons, Ltd.
Authors: Elisabete M C Alexandre; Paula Araújo; Maria F Duarte; Victor de Freitas; Manuela Pintado; Jorge A Saraiva Journal: J Food Sci Technol Date: 2017-05-26 Impact factor: 2.701
Authors: Noura M Mesalam; Sami Ali Aldhumri; Salah A Gabr; Marwa A Ibrahim; Asmaa K Al-Mokaddem; Abdel-Moneim Eid Abdel-Moneim Journal: Mol Biol Rep Date: 2021-07-09 Impact factor: 2.316
Authors: Sydney E Schnur; Raghavendra G Amachawadi; Giovanna Baca; Sarah Sexton-Bowser; Davina H Rhodes; Dmitriy Smolensky; Thomas J Herald; Ramasamy Perumal; Daniel U Thomson; Tiruvoor G Nagaraja Journal: Antibiotics (Basel) Date: 2021-05-17