BACKGROUND: Retinal argon laser coagulation is an established procedure for induction of choroidal neovascularization (CNV) in rodents. This study aimed to evaluate the in-vivo and ex-vivo morphology and variability of laser-induced CNV spots over time. METHODS: Female C57/Bl/6 mice, 3-6 months of age, were treated with five spots of retinal argon laser coagulation per eye (150 mW, 100 ms, 50 μm). In-vivo fluorescein angiography (FA) and standard high-resolution spectral-domain optical coherence tomography (SD-OCT) were performed on day (d) 0, d1, d4, d7, d14 and d21. Ex-vivo histology, CD31 immunostaining, flatmount and confocal microscopy were also conducted. CNV size in the retinal and choroidal focus, CNV morphology, central retinal thickness (CRT) and FA CNV activity grading were assessed in-vivo at all times and compared to the ex-vivo assessments. RESULTS: SD-OCT revealed sub-retinal and intra-retinal fluid, and permitted evaluation of longitudinal morphologic changes of the induced CNV. Laser spot area in FA and CRT in SD-OCT did not differ in longitudinal evaluation. CNV could not be consistently outlined on SD-OCT images, and CNV volume as assessed on SD-OCT did not change over time. Significant CNV activity changes were only found in FA CNV activity grading, peaking on d4 and decreasing by d7. CONCLUSIONS: Non-invasive SD-OCT provides additional morphological information on laser-induced CNV. However, reliable evaluation of CNV requires FA. Spontaneous regression of CNV activity within 14 days after induction has to be taken into account when utilizing this model for testing the efficacies of potential future treatments.
BACKGROUND:Retinal argon laser coagulation is an established procedure for induction of choroidal neovascularization (CNV) in rodents. This study aimed to evaluate the in-vivo and ex-vivo morphology and variability of laser-induced CNV spots over time. METHODS: Female C57/Bl/6 mice, 3-6 months of age, were treated with five spots of retinal argon laser coagulation per eye (150 mW, 100 ms, 50 μm). In-vivo fluorescein angiography (FA) and standard high-resolution spectral-domain optical coherence tomography (SD-OCT) were performed on day (d) 0, d1, d4, d7, d14 and d21. Ex-vivo histology, CD31 immunostaining, flatmount and confocal microscopy were also conducted. CNV size in the retinal and choroidal focus, CNV morphology, central retinal thickness (CRT) and FA CNV activity grading were assessed in-vivo at all times and compared to the ex-vivo assessments. RESULTS:SD-OCT revealed sub-retinal and intra-retinal fluid, and permitted evaluation of longitudinal morphologic changes of the induced CNV. Laser spot area in FA and CRT in SD-OCT did not differ in longitudinal evaluation. CNV could not be consistently outlined on SD-OCT images, and CNV volume as assessed on SD-OCT did not change over time. Significant CNV activity changes were only found in FA CNV activity grading, peaking on d4 and decreasing by d7. CONCLUSIONS: Non-invasive SD-OCT provides additional morphological information on laser-induced CNV. However, reliable evaluation of CNV requires FA. Spontaneous regression of CNV activity within 14 days after induction has to be taken into account when utilizing this model for testing the efficacies of potential future treatments.
Authors: Gesine Huber; Susanne C Beck; Christian Grimm; Ayse Sahaboglu-Tekgoz; Francois Paquet-Durand; Andreas Wenzel; Peter Humphries; T Michael Redmond; Mathias W Seeliger; M Dominik Fischer Journal: Invest Ophthalmol Vis Sci Date: 2009-08-06 Impact factor: 4.799
Authors: Michelle L Gabriele; Hiroshi Ishikawa; Joel S Schuman; Richard A Bilonick; Jongsick Kim; Larry Kagemann; Gadi Wollstein Journal: Invest Ophthalmol Vis Sci Date: 2010-06-23 Impact factor: 4.799
Authors: Andrea Giani; Aristomenis Thanos; Mi In Roh; Edward Connolly; George Trichonas; Ivana Kim; Evangelos Gragoudas; Demetrios Vavvas; Joan W Miller Journal: Invest Ophthalmol Vis Sci Date: 2011-06-01 Impact factor: 4.799
Authors: Serge Resnikoff; Donatella Pascolini; Daniel Etya'ale; Ivo Kocur; Ramachandra Pararajasegaram; Gopal P Pokharel; Silvio P Mariotti Journal: Bull World Health Organ Date: 2004-12-14 Impact factor: 9.408
Authors: Hyeong Gon Yu; Xiaoqing Liu; Szilard Kiss; Edward Connolly; Evangelos S Gragoudas; Norman A Michaud; Oleg V Bulgakov; Michael Adamian; Margaret M DeAngelis; Joan W Miller; Tiansen Li; Ivana K Kim Journal: Invest Ophthalmol Vis Sci Date: 2008-02-22 Impact factor: 4.799
Authors: Rania S Sulaiman; Judith Quigley; Xiaoping Qi; Michael N O'Hare; Maria B Grant; Michael E Boulton; Timothy W Corson Journal: J Ocul Pharmacol Ther Date: 2015-06-10 Impact factor: 2.671