C Unger1, C Lück. 1. Institute of Medical Microbiology and Hygiene, Faculty of Medicine 'Carl Gustav Carus', University of Technology, Dresden, Germany.
Abstract
AIMS: We undertook a series of experiments to investigate the susceptibility of Legionella pneumophila grown under extracellular and intracellular conditions and other water-related bacteria to silver ions. METHODS AND RESULTS: In this study, the antimicrobial effect of silver ions to intra- and extra-cellular grown Legionella bacteria was investigated. The minimal inhibitory concentration (MIC) after 24 h exposure, leading to a 5 log reduction, was c. 64 μg l(-1) AgNO(3) for extracellular grown Legionella and other tested Gram-positive and Gram-negative bacteria. In contrast, the MIC for intracellularly grown Legionella was up to 4096 μg l(-1) AgNO(3) after 24 h. Furthermore, the heterotrophic bacteria grown within a biofilm model were killed at a concentration of 4-16 μg l(-1) AgNO(3). In contrast, biofilm-associated Legionella were less sensitive (MIC 128-512 μg l(-1) AgNO(3)). CONCLUSION: Intracellularly and biofilm-grown legionellae are less sensitive against silver compared with agar-grown bacteria. SIGNIFICANCE AND IMPACT OF THE STUDY: The reduced sensitivity of Legionella grown in amoebae might explain why the effect of silver decontamination requires an extended exposure in field trials.
AIMS: We undertook a series of experiments to investigate the susceptibility of Legionella pneumophila grown under extracellular and intracellular conditions and other water-related bacteria to silver ions. METHODS AND RESULTS: In this study, the antimicrobial effect of silver ions to intra- and extra-cellular grown Legionella bacteria was investigated. The minimal inhibitory concentration (MIC) after 24 h exposure, leading to a 5 log reduction, was c. 64 μg l(-1) AgNO(3) for extracellular grown Legionella and other tested Gram-positive and Gram-negative bacteria. In contrast, the MIC for intracellularly grown Legionella was up to 4096 μg l(-1) AgNO(3) after 24 h. Furthermore, the heterotrophic bacteria grown within a biofilm model were killed at a concentration of 4-16 μg l(-1) AgNO(3). In contrast, biofilm-associated Legionella were less sensitive (MIC 128-512 μg l(-1) AgNO(3)). CONCLUSION: Intracellularly and biofilm-grown legionellae are less sensitive against silver compared with agar-grown bacteria. SIGNIFICANCE AND IMPACT OF THE STUDY: The reduced sensitivity of Legionella grown in amoebae might explain why the effect of silver decontamination requires an extended exposure in field trials.