Ginat Narkis1, Itai Tzchori, Tsadok Cohen, Alex Holtz, Eric Wier, Heiner Westphal. 1. Laboratory of Mammalian Genes and Development, Program in Genomics of Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA. ginatnarkis@gmail.com
Abstract
BACKGROUND: The developing limb has served as an excellent model for studying pattern formation and signal transduction in mammalians. Many of the crucial genes that regulate growth and patterning of the limb following limb bud formation are now well known. However, details regarding the control of limb initiation and early stages of outgrowth remain to be defined. This report is focused on genetic events that pave the way for the establishment of a hindlimb bud. RESULTS: Fgf10 and Tbx are crucial for early phases of limb bud initiation. Here we show that in the absence of Isl1 or of Ldb1/2, there is no hindlimb bud development. Fgf10 expression in the bud mesenchyme is dependent on Isl1 and its Ldb co-regulators. CONCLUSIONS: Thus, Isl1 and the Ldb co-regulators of transcription are essential early determinants of mouse limb development. Isl1/Ldb complexes regulate Fgf10 to orchestrate the earliest stages of hindlimb formation.
BACKGROUND: The developing limb has served as an excellent model for studying pattern formation and signal transduction in mammalians. Many of the crucial genes that regulate growth and patterning of the limb following limb bud formation are now well known. However, details regarding the control of limb initiation and early stages of outgrowth remain to be defined. This report is focused on genetic events that pave the way for the establishment of a hindlimb bud. RESULTS:Fgf10 and Tbx are crucial for early phases of limb bud initiation. Here we show that in the absence of Isl1 or of Ldb1/2, there is no hindlimb bud development. Fgf10 expression in the bud mesenchyme is dependent on Isl1 and its Ldb co-regulators. CONCLUSIONS: Thus, Isl1 and the Ldb co-regulators of transcription are essential early determinants of mouse limb development. Isl1/Ldb complexes regulate Fgf10 to orchestrate the earliest stages of hindlimb formation.
Authors: Pooja Agarwal; John N Wylie; Juan Galceran; Oksana Arkhitko; Cuiling Li; Chuxia Deng; Rudolf Grosschedl; Benoit G Bruneau Journal: Development Date: 2003-02 Impact factor: 6.868
Authors: Mahua Mukhopadhyay; Andreas Teufel; Tsuyoshi Yamashita; Alan D Agulnick; Lan Chen; Karen M Downs; Alice Schindler; Alexander Grinberg; Sing-Ping Huang; David Dorward; Heiner Westphal Journal: Development Date: 2003-02 Impact factor: 6.868
Authors: Sofia Nasif; Flavio S J de Souza; Laura E González; Miho Yamashita; Daniela P Orquera; Malcolm J Low; Marcelo Rubinstein Journal: Proc Natl Acad Sci U S A Date: 2015-03-30 Impact factor: 11.205
Authors: Saunders T Ching; Carlos R Infante; Wen Du; Amnon Sharir; Sungdae Park; Douglas B Menke; Ophir D Klein Journal: Hum Mol Genet Date: 2018-01-01 Impact factor: 6.150
Authors: Yangu Zhao; Pierre Flandin; Daniel Vogt; Alexander Blood; Edit Hermesz; Heiner Westphal; John L R Rubenstein Journal: Dev Biol Date: 2013-10-21 Impact factor: 3.582
Authors: Elena F Boer; Hannah F Van Hollebeke; Sungdae Park; Carlos R Infante; Douglas B Menke; Michael D Shapiro Journal: Dev Biol Date: 2019-06-24 Impact factor: 3.582
Authors: Ryutaro Akiyama; Hiroko Kawakami; M Mark Taketo; Sylvia M Evans; Naoyuki Wada; Anna Petryk; Yasuhiko Kawakami Journal: Dev Biol Date: 2014-01-11 Impact factor: 3.582