Xiao-lei Wang1, Xi-mei Chen, Jian-ping Fang, Chang-qin Yang. 1. Department of Gastroenterology, Institute of Digestive Disease, Tongji Hospital affiliated to Tongji University, Shanghai, China. xlwang2006@163.com
Abstract
AIM: To investigate the expression of c-Met in peritoneal free cancer cells isolated from human gastric cancer ascites, and its relationship to peritoneal dissemination of gastric cancer. METHODS: Peritoneal free cancer cells (PFCCs) were isolated from ascites specimens of gastric cancer patients. c-Met expression in PFCCs was detected with immunocytochemistry. In human gastric cancer cell line SGC7901, c-Met expression was detected using RT-PCR and Western blot, and was suppressed with lentivirus-mediated RNAi. The proliferation of SGC7901 cells was measured using MTT assay, and the invasion ability was detected with invasion assay. The adhesion of SGC7901 cells to peritoneum was observed in human peritoneal mesothelial cells (HPMCs) monolayer in vitro and in mice in vivo. RESULTS: PFCCs were isolated from ascites of 6 out of 10 gastric cancer patients. c-Met expression in PFCCs was detected in 5 of the 6 gastric cancer patients. In SGC7901 cells, Lentivirus-mediated RNAi significantly reduced both c-Met mRNA and protein expression, which resulted in suppressing the cell proliferation, invasion and adhesion to peritoneum. The expression of α3β1 integrin and E-cadherin was significantly inhibited in SGC7901 cells transfected with Lenti-miRNAc-Met. In the peritoneal dissemination model of gastric cancer, intraperitoneal injection of Lenti-miRNAc-Met markedly suppressed the tumor Progression of SGC7901 cells. CONCLUSION: c-Met is expressed in PFCCs from the ascites of gastric cancer patients. Down-regulation of c-Met expression markedly suppresses the multistep process of peritoneal dissemination, thus may be a potential target for the treatment of gastric cancer.
AIM: To investigate the expression of c-Met in peritoneal free cancer cells isolated from humangastric cancer ascites, and its relationship to peritoneal dissemination of gastric cancer. METHODS: Peritoneal free cancer cells (PFCCs) were isolated from ascites specimens of gastric cancerpatients. c-Met expression in PFCCs was detected with immunocytochemistry. In humangastric cancer cell line SGC7901, c-Met expression was detected using RT-PCR and Western blot, and was suppressed with lentivirus-mediated RNAi. The proliferation of SGC7901 cells was measured using MTT assay, and the invasion ability was detected with invasion assay. The adhesion of SGC7901 cells to peritoneum was observed in human peritoneal mesothelial cells (HPMCs) monolayer in vitro and in mice in vivo. RESULTS: PFCCs were isolated from ascites of 6 out of 10 gastric cancerpatients. c-Met expression in PFCCs was detected in 5 of the 6 gastric cancerpatients. In SGC7901 cells, Lentivirus-mediated RNAi significantly reduced both c-Met mRNA and protein expression, which resulted in suppressing the cell proliferation, invasion and adhesion to peritoneum. The expression of α3β1 integrin and E-cadherin was significantly inhibited in SGC7901 cells transfected with Lenti-miRNAc-Met. In the peritoneal dissemination model of gastric cancer, intraperitoneal injection of Lenti-miRNAc-Met markedly suppressed the tumor Progression of SGC7901 cells. CONCLUSION:c-Met is expressed in PFCCs from the ascites of gastric cancerpatients. Down-regulation of c-Met expression markedly suppresses the multistep process of peritoneal dissemination, thus may be a potential target for the treatment of gastric cancer.
Authors: Véronique Orian-Rousseau; Linfeng Chen; Jonathan P Sleeman; Peter Herrlich; Helmut Ponta Journal: Genes Dev Date: 2002-12-01 Impact factor: 11.361
Authors: J H Clement; M Schwalbe; N Buske; K Wagner; M Schnabelrauch; P Görnert; K O Kliche; K Pachmann; W Weitschies; K Höffken Journal: J Cancer Res Clin Oncol Date: 2006-01-24 Impact factor: 4.553
Authors: S D Barker; E Casado; J Gomez-Navarro; J Xiang; W Arafat; P Mahasreshti; T B Pustilnik; A Hemminki; G P Siegal; R D Alvarez; D T Curiel Journal: Gynecol Oncol Date: 2001-07 Impact factor: 5.482
Authors: Kenjiro Sawada; A Reza Radjabi; Nariyoshi Shinomiya; Emily Kistner; Hilary Kenny; Amy R Becker; Muge A Turkyilmaz; Ravi Salgia; S Diane Yamada; George F Vande Woude; Maria S Tretiakova; Ernst Lengyel Journal: Cancer Res Date: 2007-02-15 Impact factor: 12.701
Authors: Yong Sang Hong; Jihun Kim; Eirini Pectasides; Cameron Fox; Seung-Woo Hong; Qiuping Ma; Gabrielle S Wong; Shouyong Peng; Matthew D Stachler; Aaron R Thorner; Paul Van Hummelen; Adam J Bass Journal: PLoS One Date: 2014-10-28 Impact factor: 3.240