Literature DB >> 22403425

A novel δ-hemolysis screening method for detecting heteroresistant vancomycin-intermediate Staphylococcus aureus and vancomycin-intermediate S. aureus.

V Cafiso1, T Bertuccio, D Spina, S Purrello, G Blandino, Stefania Stefani.   

Abstract

We assessed a new screening method, based on δ-hemolysin production in the presence of 6 mg/liter vancomycin, to distinguish heteroresistant vancomycin-intermediate Staphylococcus aureus (hVISA) and vancomycin-intermediate S. aureus (VISA) from vancomycin-susceptible S. aureus (VSSA). On 37 clinical methicillin-resistant S. aureus (MRSA) isolates, hVISA and VISA displayed no δ-hemolysis whereas VSSA displayed strong δ-hemolysis, showing 91.6% sensitivity. These data, supported by real-time reverse transcription PCR (real-time RT-PCR) highlighting an hld downregulation, i.e., VSSA>hVISA>VISA, define this new assay as a valid screening method.

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Year:  2012        PMID： 22403425      PMCID： PMC3347129          DOI： 10.1128/JCM.06307-11

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  15 in total

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2.  Isolates with low-level vancomycin resistance associated with persistent methicillin-resistant Staphylococcus aureus bacteremia.

Authors:  Benjamin P Howden; Paul D R Johnson; Peter B Ward; Timothy P Stinear; John K Davies
Journal:  Antimicrob Agents Chemother       Date:  2006-09       Impact factor: 5.191

Review 3.  Adaptation of methicillin-resistant Staphylococcus aureus in the face of vancomycin therapy.

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4.  agr-Genotyping and transcriptional analysis of biofilm-producing Staphylococcus aureus.

Authors:  Viviana Cafiso; Taschia Bertuccio; Maria Santagati; Vanessa Demelio; Daniela Spina; Giuseppe Nicoletti; Stefania Stefani
Journal:  FEMS Immunol Med Microbiol       Date:  2007-10

5.  Staphylococcus aureus accessory gene regulator (agr) group II: is there a relationship to the development of intermediate-level glycopeptide resistance?

Authors:  George Sakoulas; George M Eliopoulos; Robert C Moellering; Richard P Novick; Lata Venkataraman; Christine Wennersten; Paola C DeGirolami; Mitchell J Schwaber; Howard S Gold
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6.  Accessory gene regulator (agr) locus in geographically diverse Staphylococcus aureus isolates with reduced susceptibility to vancomycin.

Authors:  George Sakoulas; George M Eliopoulos; Robert C Moellering; Christine Wennersten; Lata Venkataraman; Richard P Novick; Howard S Gold
Journal:  Antimicrob Agents Chemother       Date:  2002-05       Impact factor: 5.191

7.  Community- and health care-associated methicillin-resistant Staphylococcus aureus: a comparison of molecular epidemiology and antimicrobial activities of various agents.

Authors:  Brian T Tsuji; Michael J Rybak; Chrissy M Cheung; Muhammad Amjad; Glenn W Kaatz
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Authors:  F Campanile; D Bongiorno; M Falcone; F Vailati; M B Pasticci; M Perez; A Raglio; F Rumpianesi; C Scuderi; F Suter; M Venditti; C Venturelli; V Ravasio; M Codeluppi; S Stefani
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2011-08-07       Impact factor: 3.267

10.  Hospital-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) in Italy.

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  5 in total

1.  Methicillin-Resistant Staphylococcus aureus Grown on Vancomycin-Supplemented Screening Agar Displays Enhanced Biofilm Formation.

Authors:  Wenjiao Chang; Ding Ding; Shanshan Zhang; Yuanyuan Dai; Qing Pan; Huaiwei Lu; Qingli Luo; Jilong Shen; Xiaoling Ma
Journal:  Antimicrob Agents Chemother       Date:  2015-10-12       Impact factor: 5.191

2.  In vivo development of daptomycin resistance in vancomycin-susceptible methicillin-resistant Staphylococcus aureus severe infections previously treated with glycopeptides.

Authors:  A Capone; V Cafiso; F Campanile; G Parisi; B Mariani; N Petrosillo; S Stefani
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2016-01-27       Impact factor: 3.267

3.  Phenotypic and Molecular Characterisation of Staphylococcus Aureus with Reduced Vancomycin Susceptibility Derivated in Vitro.

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Journal:  Open Med (Wars)       Date:  2018-10-22

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Journal:  PLoS One       Date:  2012-07-06       Impact factor: 3.240

5.  Clinical outcomes and molecular typing of heterogenous vancomycin-intermediate Staphylococcus aureus bacteremia in patients in intensive care units.

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  5 in total

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