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Literature DB >> 22403418

Real-time PCR assay for identification of Blastomyces dermatitidis in culture and in tissue.

Ketevan Sidamonidze¹, Marcia K Peck, Michael Perez, Dennis Baumgardner, Gerald Smith, Vishnu Chaturvedi, Sudha Chaturvedi.

Abstract

The TaqMan real-time PCR assay was developed from the Blastomyces dermatitidis BAD1 gene promoter. The assay identified all haplotypes of B. dermatitidis and five of six positive paraffin-embedded tissues. The assay sensitivity threshold was 1 pg genomic DNA of the mold form and 2 CFU of the yeast form of B. dermatitidis. No cross-reactivity was observed against other fungal DNA. The assay allowed rapid (5-h) identification of B. dermatitidis from culture and from clinical specimens.

Entities: Chemical Species

Mesh：

Substances：
DNA, Fungal

Year: 2012 PMID： 22403418 PMCID： PMC3347106 DOI： 10.1128/JCM.00310-12

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

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10. Genomic cloning, characterization, and functional analysis of the major surface adhesin WI-1 on Blastomyces dermatitidis yeasts.

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Journal: J Biol Chem Date: 1995-12-22 Impact factor: 5.157

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