AIM: Unearthing of silenced genes in colorectal cancer (CRC). MATERIALS AND METHODS: Oligonucleotide microarray was used in order to find changes in gene expression in five CRC cell lines before and after 5-aza-2'-Deoxycitidine treatment. Up-regulated genes were integrated with expression profile of matched colorectal tissue samples. Methylation-specific polymerase chain reaction and Real-time quantitative reverse transcription polymerase chain reaction were used to further analyze candidates using 15 CRC cell lines and 23 paired samples. RESULTS: After applying study selection criteria for 68 genes obtained from integrated arrays, we identified 16 genes; apoptosis-stimulating of p53 protein 1(ASPP1) and Scavenger receptor class A, member 5 (SCARA5) were selected for further analysis. Methylation was only identified for SCARA5 in 20% of the cell lines and in 17% of tumor the samples. Down expression of SCARA5 was observed in CRC cell lines and in tumor samples compared to normal (p<0.001 and p=0.001, respectively). CONCLUSION: Genome-wide screening identifies genes potentially affected by methylation in CRC. SCARA5 may have a role in tumorigenesis in CRC.
AIM: Unearthing of silenced genes in colorectal cancer (CRC). MATERIALS AND METHODS:Oligonucleotide microarray was used in order to find changes in gene expression in five CRC cell lines before and after 5-aza-2'-Deoxycitidine treatment. Up-regulated genes were integrated with expression profile of matched colorectal tissue samples. Methylation-specific polymerase chain reaction and Real-time quantitative reverse transcription polymerase chain reaction were used to further analyze candidates using 15 CRC cell lines and 23 paired samples. RESULTS: After applying study selection criteria for 68 genes obtained from integrated arrays, we identified 16 genes; apoptosis-stimulating of p53 protein 1(ASPP1) and Scavenger receptor class A, member 5 (SCARA5) were selected for further analysis. Methylation was only identified for SCARA5 in 20% of the cell lines and in 17% of tumor the samples. Down expression of SCARA5 was observed in CRC cell lines and in tumor samples compared to normal (p<0.001 and p=0.001, respectively). CONCLUSION: Genome-wide screening identifies genes potentially affected by methylation in CRC. SCARA5 may have a role in tumorigenesis in CRC.
Authors: Orsolya Galamb; Alexandra Kalmár; Bálint Péterfia; István Csabai; András Bodor; Dezső Ribli; Tibor Krenács; Árpád V Patai; Barnabás Wichmann; Barbara Kinga Barták; Kinga Tóth; Gábor Valcz; Sándor Spisák; Zsolt Tulassay; Béla Molnár Journal: Epigenetics Date: 2016-05-31 Impact factor: 4.528
Authors: De Qing Wu; Zi Feng Yang; Ke Jian Wang; Xing Yu Feng; Ze Jian Lv; Yong Li; Zhi Xiang Jian Journal: Am J Cancer Res Date: 2018-02-01 Impact factor: 6.166
Authors: Lisa van der Lely; Janine Häfliger; Ana Montalban-Arques; Katharina Bäbler; Marlene Schwarzfischer; Max Sabev; Claudia Gottier; Silvia Lang; Michael Scharl; Marianne R Spalinger Journal: Inflamm Intest Dis Date: 2019-09-17