AIMS: To investigate the optimum conditions for the differentiation of a mouse embryonic stem cell line towards corneal epithelial cell fate. MATERIALS & METHODS: The effect of conditioned media from both metabolically active (to produce lineage A) and growth-arrested limbal fibroblasts (lineage G) were compared with basal media (lineage N) in terms of morphology and marker expression, assessed by immunocytochemistry and reverse transcription PCR. Cultures were transplanted into a porcine ex vivo model to investigate their ability for wound healing and cornea repair. RESULTS: Lineage N exhibited cobblestone morphology and expressed CK12 and p63α, while OCT4 and SSEA1 were downregulated. Post-transplantation, these cells were able to multilayer and heal after wounding while maintaining marker expression. CONCLUSION: Lineages with corneal epithelial-like characteristics, which are derived from embryonic stem cells, have potential for use in the study of corneal wound healing and therapy.
AIMS: To investigate the optimum conditions for the differentiation of a mouse embryonic stem cell line towards corneal epithelial cell fate. MATERIALS & METHODS: The effect of conditioned media from both metabolically active (to produce lineage A) and growth-arrested limbal fibroblasts (lineage G) were compared with basal media (lineage N) in terms of morphology and marker expression, assessed by immunocytochemistry and reverse transcription PCR. Cultures were transplanted into a porcine ex vivo model to investigate their ability for wound healing and cornea repair. RESULTS: Lineage N exhibited cobblestone morphology and expressed CK12 and p63α, while OCT4 and SSEA1 were downregulated. Post-transplantation, these cells were able to multilayer and heal after wounding while maintaining marker expression. CONCLUSION: Lineages with corneal epithelial-like characteristics, which are derived from embryonic stem cells, have potential for use in the study of corneal wound healing and therapy.
Authors: Dhruv Sareen; Mehrnoosh Saghizadeh; Loren Ornelas; Michael A Winkler; Kavita Narwani; Anais Sahabian; Vincent A Funari; Jie Tang; Lindsay Spurka; Vasu Punj; Ezra Maguen; Yaron S Rabinowitz; Clive N Svendsen; Alexander V Ljubimov Journal: Stem Cells Transl Med Date: 2014-07-28 Impact factor: 6.940
Authors: Ricardo P Casaroli-Marano; Núria Nieto-Nicolau; Eva M Martínez-Conesa; Michael Edel; Ana B Álvarez-Palomo Journal: J Clin Med Date: 2015-02-12 Impact factor: 4.241