AIMS: We aimed to study the interactions between human mesenchymal stem cells and the bone integration of nanostructured titanium implants. MATERIALS & METHODS: Nanopores of 20, 30 and 50 nm were prepared by anodization of titanium at 5, 10 and 20 V in a mixture of fluorhydric and acetic acid. Ti 30 and 50 nanostructures promoted early osteoblastic gene differentiation of the human mesenchymal stem cells without osteogenic supplements. The osseointegration of nanostructured and control titanium implants was compared by implantation in rat tibias for 1 and 3 weeks. RESULTS: The nanostructures significantly accelerated bone apposition and bone bonding strength in vivo in correlation with in vitro results. CONCLUSION: These findings demonstrate that specific nanostructures controlled the differentiation of cells and, thus, the integration of implants in tissues. These nanoporous titanium surfaces may be of considerable interest for dental and orthopedic implants.
AIMS: We aimed to study the interactions between human mesenchymal stem cells and the bone integration of nanostructured titanium implants. MATERIALS & METHODS: Nanopores of 20, 30 and 50 nm were prepared by anodization of titanium at 5, 10 and 20 V in a mixture of fluorhydric and acetic acid. Ti 30 and 50 nanostructures promoted early osteoblastic gene differentiation of the human mesenchymal stem cells without osteogenic supplements. The osseointegration of nanostructured and control titanium implants was compared by implantation in rat tibias for 1 and 3 weeks. RESULTS: The nanostructures significantly accelerated bone apposition and bone bonding strength in vivo in correlation with in vitro results. CONCLUSION: These findings demonstrate that specific nanostructures controlled the differentiation of cells and, thus, the integration of implants in tissues. These nanoporous titanium surfaces may be of considerable interest for dental and orthopedic implants.
Authors: Giuseppe Maria de Peppo; Hossein Agheli; Camilla Karlsson; Karin Ekström; Helena Brisby; Maria Lennerås; Stefan Gustafsson; Peter Sjövall; Anna Johansson; Eva Olsson; Jukka Lausmaa; Peter Thomsen; Sarunas Petronis Journal: Int J Nanomedicine Date: 2014-05-22
Authors: Laila Damiati; Marcus G Eales; Angela H Nobbs; Bo Su; Penelope M Tsimbouri; Manuel Salmeron-Sanchez; Matthew J Dalby Journal: J Tissue Eng Date: 2018-08-02 Impact factor: 7.813
Authors: Dimitrios Karazisis; Ahmed M Ballo; Sarunas Petronis; Hossein Agheli; Lena Emanuelsson; Peter Thomsen; Omar Omar Journal: Int J Nanomedicine Date: 2016-04-01
Authors: Vitali Goriainov; Richard B Cook; James W Murray; John C Walker; Douglas G Dunlop; Adam T Clare; Richard O C Oreffo Journal: Front Bioeng Biotechnol Date: 2018-07-24