Literature DB >> 22391535

Functional characterization of a Cassette-specific promoter in the class 1 integron-associated qnrVC1 gene.

Érica Lourenço da Fonseca1, Ana Carolina Paulo Vicente.   

Abstract

Integrons are natural expression vectors due to the presence of an intrinsic promoter (P(c)). Although rare, gene cassettes can harbor their own promoter. This study determined the functionality of an internal promoter in the qnrVC1 cassette whose presence was suggested by a level of transcription similar to that of the preceding cassette (aadA2) and confirmed by in silico analysis. Its functionality was determined by 5' rapid amplification of cDNA ends (RACE) and cloning into promoter-probe vectors. P(qnrVC) was found in the qnrVC cassette family, stressing its role in contributing to resistance manifestation.

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Year:  2012        PMID： 22391535      PMCID： PMC3370728          DOI： 10.1128/AAC.00113-12

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  14 in total

1.  Sequence alignment kernel for recognition of promoter regions.

Authors:  Leo Gordon; Alexey Ya Chervonenkis; Alex J Gammerman; Ilham A Shahmuradov; Victor V Solovyev
Journal:  Bioinformatics       Date:  2003-10-12       Impact factor: 6.937

2.  Erythromycin esterase gene ere(A) is located in a functional gene cassette in an unusual class 2 integron.

Authors:  Latefa Biskri; Didier Mazel
Journal:  Antimicrob Agents Chemother       Date:  2003-10       Impact factor: 5.191

3.  Characterization of the nonenzymatic chloramphenicol resistance (cmlA) gene of the In4 integron of Tn1696: similarity of the product to transmembrane transport proteins.

Authors:  L Bissonnette; S Champetier; J P Buisson; P H Roy
Journal:  J Bacteriol       Date:  1991-07       Impact factor: 3.490

4.  Sequence analysis of the inducible chloramphenicol resistance determinant in the Tn1696 integron suggests regulation by translational attenuation.

Authors:  H W Stokes; R M Hall
Journal:  Plasmid       Date:  1991-07       Impact factor: 3.466

5.  A novel family of potentially mobile DNA elements encoding site-specific gene-integration functions: integrons.

Authors:  H W Stokes; R M Hall
Journal:  Mol Microbiol       Date:  1989-12       Impact factor: 3.501

6.  Non-canonical sequence elements in the promoter structure. Cluster analysis of promoters recognized by Escherichia coli RNA polymerase.

Authors:  O N Ozoline; A A Deev; M V Arkhipova
Journal:  Nucleic Acids Res       Date:  1997-12-01       Impact factor: 16.971

7.  Characterization of In53, a class 1 plasmid- and composite transposon-located integron of Escherichia coli which carries an unusual array of gene cassettes.

Authors:  T Naas; Y Mikami; T Imai; L Poirel; P Nordmann
Journal:  J Bacteriol       Date:  2001-01       Impact factor: 3.490

8.  Pc promoter from class 2 integrons and the cassette transcription pattern it evokes.

Authors:  Érica Lourenço da Fonseca; Fernanda dos Santos Freitas; Ana Carolina Paulo Vicente
Journal:  J Antimicrob Chemother       Date:  2011-02-07       Impact factor: 5.790

9.  Characterization of In40 of Enterobacter aerogenes BM2688, a class 1 integron with two new gene cassettes, cmlA2 and qacF.

Authors:  M C Ploy; P Courvalin; T Lambert
Journal:  Antimicrob Agents Chemother       Date:  1998-10       Impact factor: 5.191

10.  New qnr gene cassettes associated with superintegron repeats in Vibrio cholerae O1.

Authors:  Erica L Fonseca; Fernanda Dos Santos Freitas; Verônica V Vieira; Ana C P Vicente
Journal:  Emerg Infect Dis       Date:  2008-07       Impact factor: 6.883
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  5 in total

Review 1.  Integrons: past, present, and future.

Authors:  Michael R Gillings
Journal:  Microbiol Mol Biol Rev       Date:  2014-06       Impact factor: 11.056

2.  IMP-1 encoded by a novel Tn402-like class 1 integron in clinical Achromobacter xylosoxidans, China.

Authors:  Zhenhong Chen; Haihong Fang; Li Wang; Fengjun Sun; Yong Wang; Zhe Yin; Huiying Yang; Wenhui Yang; Jie Wang; Peiyuan Xia; Dongsheng Zhou; Changting Liu
Journal:  Sci Rep       Date:  2014-11-27       Impact factor: 4.379

3.  Efficiency of integron cassette insertion in correct orientation is ensured by the interplay of the three unpaired features of attC recombination sites.

Authors:  Aleksandra Nivina; José Antonio Escudero; Claire Vit; Didier Mazel; Céline Loot
Journal:  Nucleic Acids Res       Date:  2016-08-05       Impact factor: 16.971

4.  Characterization of Novel Integrons, In1085 and In1086, and the Surrounding Genes in Plasmids from Enterobacteriaceae, and the Role for attCaadA16 Structural Features during attI1 × attC Integration.

Authors:  Dongguo Wang; Wei Hou; Jiayu Chen; Linjun Yang; Zhihui Liu; Zhe Yin; Jiao Feng; Dongsheng Zhou
Journal:  Front Microbiol       Date:  2017-06-02       Impact factor: 5.640

Review 5.  Integron Functionality and Genome Innovation: An Update on the Subtle and Smart Strategy of Integrase and Gene Cassette Expression Regulation.

Authors:  Érica L Fonseca; Ana Carolina Vicente
Journal:  Microorganisms       Date:  2022-01-20
  5 in total

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