Literature DB >> 22391419

Redesigning the NEDD8 pathway with a bacterial genetic screen for ubiquitin-like molecule transfer.

Gurkan Guntas1, Brian Kuhlman.   

Abstract

Pathways of ubiquitin-like (UBL) molecule transfer regulate a myriad of cellular cascades. Here, we report a high-throughput assay that correlates catalytic human NEDD8 transfer to bacterial survival. The assay was utilized to screen mutant NEDD8 and NAE (NEDD8-activating enzyme) libraries to engineer a more stable NEDD8 and redesign the NEDD8-NAE interface. This approach will be useful in understanding the specificities underlying UBL pathways.
Copyright © 2012 Elsevier Ltd. All rights reserved.

Entities:  

Mesh：

Substances：

Year:  2012        PMID： 22391419      PMCID： PMC3329750          DOI： 10.1016/j.jmb.2012.02.036

Source DB:  PubMed          Journal:  J Mol Biol        ISSN： 0022-2836            Impact factor:   5.469


  21 in total

1.  Genetic analysis of the twin arginine translocator secretion pathway in bacteria.

Authors:  Matthew P DeLisa; Patrik Samuelson; Tracy Palmer; George Georgiou
Journal:  J Biol Chem       Date:  2002-05-20       Impact factor: 5.157

2.  A universal, vector-based system for nucleic acid reading-frame selection.

Authors:  Stefan Lutz; Walter Fast; Stephen J Benkovic
Journal:  Protein Eng       Date:  2002-12

3.  The structure of the APPBP1-UBA3-NEDD8-ATP complex reveals the basis for selective ubiquitin-like protein activation by an E1.

Authors:  Helen Walden; Michael S Podgorski; Danny T Huang; David W Miller; Rebecca J Howard; Daniel L Minor; James M Holton; Brenda A Schulman
Journal:  Mol Cell       Date:  2003-12       Impact factor: 17.970

4.  Folding quality control in the export of proteins by the bacterial twin-arginine translocation pathway.

Authors:  Matthew P DeLisa; Danielle Tullman; George Georgiou
Journal:  Proc Natl Acad Sci U S A       Date:  2003-04-29       Impact factor: 11.205

5.  Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8.

Authors:  Helen Walden; Michael S Podgorski; Brenda A Schulman
Journal:  Nature       Date:  2003-03-20       Impact factor: 49.962

6.  Overproduction of eukaryotic SUMO-1- and SUMO-2-conjugated proteins in Escherichia coli.

Authors:  Yasuhiro Uchimura; Makoto Nakamura; Kaoru Sugasawa; Mitsuyoshi Nakao; Hisato Saitoh
Journal:  Anal Biochem       Date:  2004-08-01       Impact factor: 3.365

7.  Functional transplantation of the sumoylation machinery into Escherichia coli.

Authors:  Mario Mencía; Víctor de Lorenzo
Journal:  Protein Expr Purif       Date:  2004-10       Impact factor: 1.650

8.  In vivo and in vitro reconstitution of Atg8 conjugation essential for autophagy.

Authors:  Yoshinobu Ichimura; Yuko Imamura; Kazuo Emoto; Masato Umeda; Takeshi Noda; Yoshinori Ohsumi
Journal:  J Biol Chem       Date:  2004-07-23       Impact factor: 5.157

9.  Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer.

Authors:  Richard N Bohnsack; Arthur L Haas
Journal:  J Biol Chem       Date:  2003-05-10       Impact factor: 5.157

10.  Generation of SUMO-1 modified proteins in E. coli: towards understanding the biochemistry/structural biology of the SUMO-1 pathway.

Authors:  Yasuhiro Uchimura; Mitsuyoshi Nakao; Hisato Saitoh
Journal:  FEBS Lett       Date:  2004-04-23       Impact factor: 4.124
View more
  2 in total

1.  A survival selection strategy for engineering synthetic binding proteins that specifically recognize post-translationally phosphorylated proteins.

Authors:  Bunyarit Meksiriporn; Morgan B Ludwicki; Erin A Stephens; Allen Jiang; Hyeon-Cheol Lee; Dujduan Waraho-Zhmayev; Lutz Kummer; Fabian Brandl; Andreas Plückthun; Matthew P DeLisa
Journal:  Nat Commun       Date:  2019-04-23       Impact factor: 14.919

2.  Site-Specific Protein Ubiquitylation Using an Engineered, Chimeric E1 Activating Enzyme and E2 SUMO Conjugating Enzyme Ubc9.

Authors:  Gaku Akimoto; Arianna P Fernandes; Jeffrey W Bode
Journal:  ACS Cent Sci       Date:  2022-02-09       Impact factor: 14.553
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.