Literature DB >> 22389310

Heterodyne detected nonlinear optical imaging in a lock-in free manner.

Mikhail N Slipchenko1, Robert A Oglesbee, Delong Zhang, Wei Wu, Ji-Xin Cheng.   

Abstract

We report a compact, cost-effective tuned amplifier for frequency-selective amplification of the modulated signal in heterodyne detected nonlinear optical microscopy. Our method improved the signal to noise ratio by an order of magnitude compared to conventional lock-in detection, as demonstrated through stimulated Raman scattering imaging of live cells and tissues at the speed of 2 μsec/pixel. Application of the tuned amplifier to transient absorption microscopy is also demonstrated. The increased signal to noise ratio allowed epi-detected in vivo imaging of myelin and blood in rat spinal cord with high spatial resolution.
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Mesh：

Year:  2012        PMID： 22389310     DOI： 10.1002/jbio.201200005

Source DB:  PubMed          Journal:  J Biophotonics        ISSN： 1864-063X            Impact factor:   3.207


  21 in total

1.  Synchronous digitization for high dynamic range lock-in amplification in beam-scanning microscopy.

Authors:  Ryan D Muir; Shane Z Sullivan; Robert A Oglesbee; Garth J Simpson
Journal:  Rev Sci Instrum       Date:  2014-03       Impact factor: 1.523

2.  Spectrally modulated stimulated Raman scattering imaging with an angle-to-wavelength pulse shaper.

Authors:  Delong Zhang; Mikhail N Slipchenko; Daniel E Leaird; Andrew M Weiner; Ji-Xin Cheng
Journal:  Opt Express       Date:  2013-06-03       Impact factor: 3.894

3.  Multimodal instrument for high-sensitivity autofluorescence and spectral optical coherence tomography of the human eye fundus.

Authors:  Katarzyna Komar; Patrycjusz Stremplewski; Marta Motoczyńska; Maciej Szkulmowski; Maciej Wojtkowski
Journal:  Biomed Opt Express       Date:  2013-10-29       Impact factor: 3.732

4.  Flexible digital signal processing architecture for narrowband and spread-spectrum lock-in detection in multiphoton microscopy and time-resolved spectroscopy.

Authors:  Jesse W Wilson; Jong Kang Park; Warren S Warren; Martin C Fischer
Journal:  Rev Sci Instrum       Date:  2015-03       Impact factor: 1.523

Review 5.  Coherent Raman Scattering Microscopy in Biology and Medicine.

Authors:  Chi Zhang; Delong Zhang; Ji-Xin Cheng
Journal:  Annu Rev Biomed Eng       Date:  2015-10-22       Impact factor: 9.590

6.  Label-free real-time imaging of myelination in the Xenopus laevis tadpole by in vivo stimulated Raman scattering microscopy.

Authors:  Chun-Rui Hu; Delong Zhang; Mikhail N Slipchenko; Ji-Xin Cheng; Bing Hu
Journal:  J Biomed Opt       Date:  2014-08       Impact factor: 3.170

Review 7.  Invited Review Article: Pump-probe microscopy.

Authors:  Martin C Fischer; Jesse W Wilson; Francisco E Robles; Warren S Warren
Journal:  Rev Sci Instrum       Date:  2016-03       Impact factor: 1.523

8.  In Situ and In Vivo Molecular Analysis by Coherent Raman Scattering Microscopy.

Authors:  Chien-Sheng Liao; Ji-Xin Cheng
Journal:  Annu Rev Anal Chem (Palo Alto Calif)       Date:  2016-06-12       Impact factor: 10.745

Review 9.  Quantitative imaging of lipid droplets in single cells.

Authors:  Anushka Gupta; Gabriel F Dorlhiac; Aaron M Streets
Journal:  Analyst       Date:  2019-01-28       Impact factor: 4.616

10.  Denoising Stimulated Raman Spectroscopic Images by Total Variation Minimization.

Authors:  Chien-Sheng Liao; Joon Hee Choi; Delong Zhang; Stanley H Chan; Ji-Xin Cheng
Journal:  J Phys Chem C Nanomater Interfaces       Date:  2015-07-29       Impact factor: 4.126
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