BACKGROUND: Over 6 million people die annually in the world because of cancer. Several groups are focused on studying cancer chemoprevention approaches. Resveratrol, a polyphenol, at high dosages, has been reported as antitumor and chemopreventive. However, it has a dose-dependent effect on cell death, even on some cancer cells. OBJECTIVES: Our aim was to investigate this dose-dependent effect on human bladder carcinoma ECV304 cells during oxidative stress condition. METHODS: For this purpose, ECV304 cells incubated with different Resveratrol concentrations were analyzed as for their metabolic rate, membrane permeability, DNA fragmentation, anti/proapoptotic protein levels and phosphatidylserine exposure after oxidative stress. RESULTS: Resveratrol induced cell death at high concentrations (>20 μM), but not at low ones (0.1-20 μM). Pretreatment with 2.5 μM protected the cells from oxidative damage, whereas 50 μM intensified the cell death and significantly increased Bad/Bcl-2 ratio (proapoptotic/antiapoptotic proteins). Resveratrol was able to modulate NO and PGE(2) secretion and performed an anti-adhesion activity of neutrophils on PMA-activated ECV304 cells. CONCLUSIONS: Resveratrol at high doses induces cell death of ECV304 cells whereas low doses induce protection. Modulation of Bcl-2 protein induced by Resveratrol could be mediating this effect. This information about the role of Resveratrol on cancer alerts us about its dose-dependent effects and could lead the design of future chemoprevention strategies. Published by Elsevier Ireland Ltd.
BACKGROUND: Over 6 million people die annually in the world because of cancer. Several groups are focused on studying cancer chemoprevention approaches. Resveratrol, a polyphenol, at high dosages, has been reported as antitumor and chemopreventive. However, it has a dose-dependent effect on cell death, even on some cancer cells. OBJECTIVES: Our aim was to investigate this dose-dependent effect on humanbladder carcinoma ECV304 cells during oxidative stress condition. METHODS: For this purpose, ECV304 cells incubated with different Resveratrol concentrations were analyzed as for their metabolic rate, membrane permeability, DNA fragmentation, anti/proapoptotic protein levels and phosphatidylserine exposure after oxidative stress. RESULTS:Resveratrol induced cell death at high concentrations (>20 μM), but not at low ones (0.1-20 μM). Pretreatment with 2.5 μM protected the cells from oxidative damage, whereas 50 μM intensified the cell death and significantly increased Bad/Bcl-2 ratio (proapoptotic/antiapoptotic proteins). Resveratrol was able to modulate NO and PGE(2) secretion and performed an anti-adhesion activity of neutrophils on PMA-activated ECV304 cells. CONCLUSIONS:Resveratrol at high doses induces cell death of ECV304 cells whereas low doses induce protection. Modulation of Bcl-2 protein induced by Resveratrol could be mediating this effect. This information about the role of Resveratrol on cancer alerts us about its dose-dependent effects and could lead the design of future chemoprevention strategies. Published by Elsevier Ireland Ltd.
Authors: Saleh A Almatroodi; Mohammed A Alsahli; Abdullah S M Aljohani; Fahad A Alhumaydhi; Ali Yousif Babiker; Amjad Ali Khan; Arshad Husain Rahmani Journal: Molecules Date: 2022-04-21 Impact factor: 4.927
Authors: Elisa Sauer; Angela M Moro; Natália Brucker; Sabrina Nascimento; Bruna Gauer; Rafael Fracasso; Adriana Gioda; Ruy Beck; José C F Moreira; Vera Lucia Eifler-Lima; Solange Cristina Garcia Journal: Int J Environ Res Public Health Date: 2014-11-13 Impact factor: 3.390
Authors: Mírian Feliciano Costa; Tais Iara Jesus; Bruno Rafael Pereira Lopes; Célio Fernando Figueiredo Angolini; Abner Montagnolli; Lorraine de Paula Gomes; Gabriela Sterle Pereira; Ana Lucia Tasca Gois Ruiz; João Ernesto Carvalho; Marcos Nogueira Eberlin; Catarina Dos Santos; Karina Alves Toledo Journal: BMC Complement Altern Med Date: 2016-10-22 Impact factor: 3.659