OBJECTIVE: To investigate the mechanism underlying sodium butyrate (NaB)-induced apoptosis of a human colon cancer cell line HCT-116. METHODS: The apoptosis of HCT-116 cells induced by NaB was confirmed by hoechst33342 staining and AnnexinV+ PI assay. The changes in the intracellular localization of stromal interaction molecule (STIM1) and Orai1 following NaB treatment were detected by immunofluorescence technique. Western blotting was used to investigate the protein expression levels of STIM1 and Orai1. RESULTS: NaB induced apoptosis and caused translocation and colocalization of STIM1 and Orai1 in HCT-116 cells. CONCLUSION: The apoptosis of human colon cancer cells induced by NaB is correlated to the redistribution of STIM1 and Orai1.
OBJECTIVE: To investigate the mechanism underlying sodium butyrate (NaB)-induced apoptosis of a humancolon cancer cell line HCT-116. METHODS: The apoptosis of HCT-116 cells induced by NaB was confirmed by hoechst33342 staining and AnnexinV+ PI assay. The changes in the intracellular localization of stromal interaction molecule (STIM1) and Orai1 following NaB treatment were detected by immunofluorescence technique. Western blotting was used to investigate the protein expression levels of STIM1 and Orai1. RESULTS:NaB induced apoptosis and caused translocation and colocalization of STIM1 and Orai1 in HCT-116 cells. CONCLUSION: The apoptosis of humancolon cancer cells induced by NaB is correlated to the redistribution of STIM1 and Orai1.