Xiang Li1, Li Pan, Bin Wang. 1. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China. lx1813@163.com
Abstract
OBJECTIVE: In the present study, we aim to evaluate the inhibitory effect of aflatoxin on Vibrio fischeri luminescence. METHODS: V. fischeri culture is treated with aflatoxin or the culture broth of aflatoxin-producing strains, and the luminescence intensity of V. fischeri is detected to analyze the influence of aflatoxin on V. fischeri. RESULTS: The logarithmic value of aflatoxin concentration and the decrease ratio of V. fischeri luminescence is in a linear relationship. Based on the regression equation between aflatoxin concentration and luminescence decrease of V. fischeri, the toxin-producing status of different microbes can be detected quickly and exactly: all of six tested Aspergillus flavus strains show toxigenicity to V. fischeri, and their toxin yield reached 14.94 mg/L - 46.45 mg/L (represented by aflatoxin concentration), while the tested Aspergillus oryzae shows no toxigenicity. CONCLUSION: The above data showed that the luminescence change of V. fischeri could exactly reflect the capability of various microbes to produce toxin (especially aflatoxin), which provided a new clue for rapid detection of aflatoxin in industrial and agricultural production and could be developed as a potential method for aflatoxin assay.
OBJECTIVE: In the present study, we aim to evaluate the inhibitory effect of aflatoxin on Vibrio fischeri luminescence. METHODS:V. fischeri culture is treated with aflatoxin or the culture broth of aflatoxin-producing strains, and the luminescence intensity of V. fischeri is detected to analyze the influence of aflatoxin on V. fischeri. RESULTS: The logarithmic value of aflatoxin concentration and the decrease ratio of V. fischeri luminescence is in a linear relationship. Based on the regression equation between aflatoxin concentration and luminescence decrease of V. fischeri, the toxin-producing status of different microbes can be detected quickly and exactly: all of six tested Aspergillus flavus strains show toxigenicity to V. fischeri, and their toxin yield reached 14.94 mg/L - 46.45 mg/L (represented by aflatoxin concentration), while the tested Aspergillus oryzae shows no toxigenicity. CONCLUSION: The above data showed that the luminescence change of V. fischeri could exactly reflect the capability of various microbes to produce toxin (especially aflatoxin), which provided a new clue for rapid detection of aflatoxin in industrial and agricultural production and could be developed as a potential method for aflatoxin assay.
Authors: Phani M Gummadidala; Yung Pin Chen; Kevin R Beauchesne; Kristen P Miller; Chandrani Mitra; Nora Banaszek; Michelle Velez-Martinez; Peter D R Moeller; John L Ferry; Alan W Decho; Anindya Chanda Journal: Front Microbiol Date: 2016-06-03 Impact factor: 5.640