OBJECTIVE: To establish a new method for the identification of Schisandra sphenanthera and S. chinensis. METHOD: Random amplified polymorphic DNA-Sequence characterized applied region (RAPD-SCAR) method was applied to screen primers. RESULT: Screening from 100 primers, only 2 random primers, which can be used to identify S. sphenanthera and S. chinensis accurately with a good reproducibility. It worked to fit them into sequence characterized applied region. CONCLUSION: RAPD-SCAR can be used to identify S. sphenanthera and S. chinensis accurately.
OBJECTIVE: To establish a new method for the identification of Schisandra sphenanthera and S. chinensis. METHOD: Random amplified polymorphic DNA-Sequence characterized applied region (RAPD-SCAR) method was applied to screen primers. RESULT: Screening from 100 primers, only 2 random primers, which can be used to identify S. sphenanthera and S. chinensis accurately with a good reproducibility. It worked to fit them into sequence characterized applied region. CONCLUSION: RAPD-SCAR can be used to identify S. sphenanthera and S. chinensis accurately.