Literature DB >> 22374809

The last step of kanamycin biosynthesis: unique deamination reaction catalyzed by the α-ketoglutarate-dependent nonheme iron dioxygenase KanJ and the NADPH-dependent reductase KanK.

Hilda Sucipto1, Fumitaka Kudo, Tadashi Eguchi.   

Abstract

Mystery solved: using heterologous expression, the activities of two enzymes exclusively belonging to the kanamycin biosynthetic pathway have been identified in vitro. A distinctive reaction mechanism to produce kanamycin is proposed and the previously unknown catalytic deamination activity of KanJ dioxygenase is uncovered.
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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Year:  2012        PMID： 22374809     DOI： 10.1002/anie.201108122

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN： 1433-7851            Impact factor:   15.336


  10 in total

1.  Genetic engineering combined with random mutagenesis to enhance G418 production in Micromonospora echinospora.

Authors:  Xianpu Ni; Zhenpeng Sun; Hongyu Zhang; Han He; Zhouxiang Ji; Huanzhang Xia
Journal:  J Ind Microbiol Biotechnol       Date:  2014-07-17       Impact factor: 3.346

2.  A study on the structure, mechanism, and biochemistry of kanamycin B dioxygenase (KanJ)-an enzyme with a broad range of substrates.

Authors:  Beata Mrugała; Anna Miłaczewska; Przemyslaw Jerzy Porebski; Ewa Niedzialkowska; Maciej Guzik; Wladek Minor; Tomasz Borowski
Journal:  FEBS J       Date:  2020-07-27       Impact factor: 5.542

3.  Assembly of a novel biosynthetic pathway for gentamicin B production in Micromonospora echinospora.

Authors:  Xianpu Ni; Zhenpeng Sun; Yawen Gu; Hao Cui; Huanzhang Xia
Journal:  Microb Cell Fact       Date:  2016-01-05       Impact factor: 5.328

4.  Modulation of kanamycin B and kanamycin A biosynthesis in Streptomyces kanamyceticus via metabolic engineering.

Authors:  Wenli Gao; Zheng Wu; Junyang Sun; Xianpu Ni; Huanzhang Xia
Journal:  PLoS One       Date:  2017-07-28       Impact factor: 3.240

Review 5.  Parallel pathways in the biosynthesis of aminoglycoside antibiotics.

Authors:  Yi Yu; Qi Zhang; Zixin Deng
Journal:  F1000Res       Date:  2017-05-18

6.  Chemoenzymatic Total Synthesis of Deoxy-, epi-, and Podophyllotoxin and a Biocatalytic Kinetic Resolution of Dibenzylbutyrolactones.

Authors:  Mattia Lazzarotto; Lucas Hammerer; Michael Hetmann; Annika Borg; Luca Schmermund; Lorenz Steiner; Peter Hartmann; Ferdinand Belaj; Wolfgang Kroutil; Karl Gruber; Michael Fuchs
Journal:  Angew Chem Int Ed Engl       Date:  2019-05-08       Impact factor: 15.336

7.  Establishment of a highly efficient conjugation protocol for Streptomyces kanamyceticus ATCC12853.

Authors:  Shuman Zhang; Tiansheng Chen; Jia Jia; Liwen Guo; Huizheng Zhang; Chao Li; Renzhong Qiao
Journal:  Microbiologyopen       Date:  2018-11-17       Impact factor: 3.139

8.  Overproduction of gentamicin B in industrial strain Micromonospora echinospora CCTCC M 2018898 by cloning of the missing genes genR and genS.

Authors:  Yingying Chang; Baozhong Chai; Yunkun Ding; Min He; Linghui Zheng; Yun Teng; Zixin Deng; Yi Yu; Tiangang Liu
Journal:  Metab Eng Commun       Date:  2019-07-24

9.  Complete reconstitution of the diverse pathways of gentamicin B biosynthesis.

Authors:  Yeon Hee Ban; Myoung Chong Song; Jae-Yeon Hwang; Hea-Lyung Shin; Hak Joong Kim; Seung Kon Hong; Na Joon Lee; Je Won Park; Sun-Shin Cha; Hung-Wen Liu; Yeo Joon Yoon
Journal:  Nat Chem Biol       Date:  2019-01-14       Impact factor: 15.040

10.  The bifunctional enzyme, GenB4, catalyzes the last step of gentamicin 3',4'-di-deoxygenation via reduction and transamination activities.

Authors:  Xiaotang Chen; Hui Zhang; Shaotong Zhou; Mingjun Bi; Shizhou Qi; Huiyuan Gao; Xianpu Ni; Huanzhang Xia
Journal:  Microb Cell Fact       Date:  2020-03-10       Impact factor: 5.328
  10 in total

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