AIM: To investigate putative biological damage caused by GSM mobile phone frequencies by assessing electromagnetic fields during mobile phone working. METHODS: Neuron-like cells, obtained by retinoic-acid-induced differentiation of human neuroblastoma SH-SY5Y cells, were exposed for 2 h and 4 h to microwaves at 1800 MHz frequency bands. RESULTS: Cell stress response was evaluated by MTT assay as well as changes in the heat shock protein expression (Hsp20, Hsp27 and Hsp70) and caspase-3 activity levels, as biomarkers of apoptotic pathway. Under our experimental conditions, neither cell viability nor Hsp27 expression nor caspase-3 activity was significantly changed. Interestingly, a significant decrease in Hsp20 expression was observed at both times of exposure, whereas Hsp70 levels were significantly increased only after 4 h exposure. CONCLUSION: The modulation of the expression of Hsps in neuronal cells can be an early response to radiofrequency microwaves.
AIM: To investigate putative biological damage caused by GSM mobile phone frequencies by assessing electromagnetic fields during mobile phone working. METHODS: Neuron-like cells, obtained by retinoic-acid-induced differentiation of humanneuroblastoma SH-SY5Y cells, were exposed for 2 h and 4 h to microwaves at 1800 MHz frequency bands. RESULTS: Cell stress response was evaluated by MTT assay as well as changes in the heat shock protein expression (Hsp20, Hsp27 and Hsp70) and caspase-3 activity levels, as biomarkers of apoptotic pathway. Under our experimental conditions, neither cell viability nor Hsp27 expression nor caspase-3 activity was significantly changed. Interestingly, a significant decrease in Hsp20 expression was observed at both times of exposure, whereas Hsp70 levels were significantly increased only after 4 h exposure. CONCLUSION: The modulation of the expression of Hsps in neuronal cells can be an early response to radiofrequency microwaves.
Authors: Alberto López-Furelos; José Manuel Leiro-Vidal; Aarón Ángel Salas-Sánchez; Francisco José Ares-Pena; María Elena López-Martín Journal: Oncotarget Date: 2016-10-04
Authors: Myrtill Simkó; Daniel Remondini; Olga Zeni; Maria Rosaria Scarfi Journal: Int J Environ Res Public Health Date: 2016-07-12 Impact factor: 3.390
Authors: Dominique Belpomme; George L Carlo; Philippe Irigaray; David O Carpenter; Lennart Hardell; Michael Kundi; Igor Belyaev; Magda Havas; Franz Adlkofer; Gunnar Heuser; Anthony B Miller; Daniela Caccamo; Chiara De Luca; Lebrecht von Klitzing; Martin L Pall; Priyanka Bandara; Yael Stein; Cindy Sage; Morando Soffritti; Devra Davis; Joel M Moskowitz; S M J Mortazavi; Martha R Herbert; Hanns Moshammer; Gerard Ledoigt; Robert Turner; Anthony Tweedale; Pilar Muñoz-Calero; Iris Udasin; Tarmo Koppel; Ernesto Burgio; André Vander Vorst Journal: Int J Mol Sci Date: 2021-07-07 Impact factor: 5.923