| Literature DB >> 22367939 |
Huili Pang1, Zhongfang Tan, Guangyong Qin, Yanping Wang, Zongwei Li, Qingsheng Jin, Yimin Cai.
Abstract
A total of 140 lactic acid bacteria (LAB) strains were isolated from corn, alfalfa, clover, sainfoin, and Indian goosegrass in the Tibetan Plateau. According to phenotypic and chemotaxonomic characteristics, 16S rDNA sequence, and recA gene PCR amplification, these LAB isolates were identified as belonging to five genera and nine species. Corn contained more LAB species than other forage crops. Leuconostoc pseudomesenteroides, Lactococcus lactis subsp. lactis, Lactobacillus brevis, and Weissella paramesenteroides were dominant members of the LAB population on alfalfa, clover, sainfoin, and Indian goosegrass, respectively. The comprehensive 16S rDNA and recA-based approach effectively described the LAB community structure of the relatively abundant LAB species distributed on different forage crops. This is the first report describing the diversity and natural populations of LAB associated with Tibetan forage crops, and most isolates grow well at or below 10°C. The results will be valuable for the future design of appropriate inoculants for silage fermentation in this very cold area.Entities:
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Year: 2012 PMID： 22367939 DOI： 10.1007/s12275-012-1284-5
Source DB: PubMed Journal: J Microbiol ISSN： 1225-8873 Impact factor: 3.422