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Literature DB >> 22366300

A comparison of two approaches to extracting Cryptosporidium DNA from human stools as measured by a real-time PCR assay.

K Elwin¹, G Robinson, S J Hadfield, H V Fairclough, M Iturriza-Gómara, R M Chalmers.

Abstract

Direct extraction of Cryptosporidium DNA from 46 stools by bead-beating, guanidine thiocyanate and silica purification provided slightly lower PCR positivity (93.5% vs. 100%) and higher threshold cycle values (mean 34.93 vs. 28.03; P=0.00) than spin-column extraction from boiled, semi-purified oocyst suspensions. However, direct extraction is cheaper, and amenable to automation. Copyright Â

Entities: Chemical Species

Mesh：

Substances：
DNA, Protozoan

Year: 2012 PMID： 22366300 DOI： 10.1016/j.mimet.2012.02.006

Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363

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Cited

12 in total

1. Cryptosporidium avium n. sp. (Apicomplexa: Cryptosporidiidae) in birds.

Authors: Nikola Holubová; Bohumil Sak; Michaela Horčičková; Lenka Hlásková; Dana Květoňová; Sarah Menchaca; John McEvoy; Martin Kváč
Journal: Parasitol Res Date: 2016-02-23 Impact factor: 2.289

Review 2. A review of the global burden, novel diagnostics, therapeutics, and vaccine targets for cryptosporidium.

Authors: William Checkley; A Clinton White; Devan Jaganath; Michael J Arrowood; Rachel M Chalmers; Xian-Ming Chen; Ronald Fayer; Jeffrey K Griffiths; Richard L Guerrant; Lizbeth Hedstrom; Christopher D Huston; Karen L Kotloff; Gagandeep Kang; Jan R Mead; Mark Miller; William A Petri; Jeffrey W Priest; David S Roos; Boris Striepen; R C Andrew Thompson; Honorine D Ward; Wesley A Van Voorhis; Lihua Xiao; Guan Zhu; Eric R Houpt
Journal: Lancet Infect Dis Date: 2014-09-29 Impact factor: 25.071

3. High-throughput multiplex quantitative polymerase chain reaction method for Giardia lamblia and Cryptosporidium species detection in stool samples.

Authors: Noora Nurminen; Rosa Juuti; Sami Oikarinen; Yue-Mei Fan; Kirsi-Maarit Lehto; Charles Mangani; Kenneth Maleta; Per Ashorn; Heikki Hyöty
Journal: Am J Trop Med Hyg Date: 2015-04-27 Impact factor: 2.345

4. Assessment of microscopic and molecular tools for the diagnosis and follow-up of cryptosporidiosis in patients at risk.

Authors: Y Le Govic; K Guyot; G Certad; A Deschildre; R Novo; C Mary; B Sendid; E Viscogliosi; L Favennec; E Dei-Cas; E Fréalle; E Dutoit
Journal: Eur J Clin Microbiol Infect Dis Date: 2015-11-26 Impact factor: 3.267

5. Multicentric evaluation of a new real-time PCR assay for quantification of Cryptosporidium spp. and identification of Cryptosporidium parvum and Cryptosporidium hominis.

Authors: C Mary; E Chapey; E Dutoit; K Guyot; L Hasseine; F Jeddi; J Menotti; C Paraud; C Pomares; M Rabodonirina; A Rieux; F Derouin
Journal: J Clin Microbiol Date: 2013-05-29 Impact factor: 5.948

6. Cryptosporidium and Giardia in Livestock in Tigray, Northern Ethiopia and Associated Risk Factors for Infection: A Cross-Sectional Study.

Authors: Tsegabirhan Kifleyohannes; Ane Nødtvedt; John James Debenham; Getachew Terefe; Lucy J Robertson
Journal: Front Vet Sci Date: 2022-01-14

A comparison of two approaches to extracting Cryptosporidium DNA from human stools as measured by a real-time PCR assay.

1. Cryptosporidium avium n. sp. (Apicomplexa: Cryptosporidiidae) in birds.

Review 2. A review of the global burden, novel diagnostics, therapeutics, and vaccine targets for cryptosporidium.

3. High-throughput multiplex quantitative polymerase chain reaction method for Giardia lamblia and Cryptosporidium species detection in stool samples.

4. Assessment of microscopic and molecular tools for the diagnosis and follow-up of cryptosporidiosis in patients at risk.

5. Multicentric evaluation of a new real-time PCR assay for quantification of Cryptosporidium spp. and identification of Cryptosporidium parvum and Cryptosporidium hominis.

6. Cryptosporidium and Giardia in Livestock in Tigray, Northern Ethiopia and Associated Risk Factors for Infection: A Cross-Sectional Study.

7. DNA extraction from protozoan oocysts/cysts in feces for diagnostic PCR.

Review 8. Cryptosporidium infection in solid organ transplantation.

9. Molecular characterization of Cryptosporidium isolates from diarrheal dairy calves in France.

Review 10. Looking for Cryptosporidium: the application of advances in detection and diagnosis.