INTRODUCTION: Environmental factors causing oxidative stress are known to be associated with asthma morbidity. The antioxidative gene NFE2L2 has been implicated in asthma development in mice models. In humans, the SNPs -617C/A and -653G/A, located at the promoter region of NFE2L2 gene, have been found associated with the susceptibility to develop diverse chronic-degenerative diseases. OBJECTIVE: To determine if there is association of the -617C/A and -653G/A NFE2L2 SNPs and childhood-onset asthma in a Mexican population. MATERIALS AND METHODS: In a case-control study 242 unrelated patients with diagnosis of asthma and 358 ethnically- and sex-matched healthy individuals were included. The -617C/A and -653G/A NFE2L2 genotyping was carried out using the TaqMan allelic discrimination assay. RESULTS: The risk allele of both polymorphisms showed a high frequency in our sample (-617A: 24% and -653A: 40%), similarly to those previously reported in Asiatic populations (-617A: 24-29% and -653A: 42-52%; p > 0.05). In contrast, the -617A allele frequency was higher than that reported in a European-African admixed population (10%, p < 0.001). The allelic and genotypic frequencies from both polymorphisms showed no significant differences among cases and controls in female and male samples. Likewise, haplotype analysis found no association between NFE2L2 gene variants and the disease. CONCLUSIONS: Despite the experimental evidence suggesting that NFE2L2 gene is involved in asthma pathogenesis, the -617C/A and -653G/A SNPs were not associated with childhood-onset asthma.
INTRODUCTION: Environmental factors causing oxidative stress are known to be associated with asthma morbidity. The antioxidative gene NFE2L2 has been implicated in asthma development in mice models. In humans, the SNPs -617C/A and -653G/A, located at the promoter region of NFE2L2 gene, have been found associated with the susceptibility to develop diverse chronic-degenerative diseases. OBJECTIVE: To determine if there is association of the -617C/A and -653G/ANFE2L2 SNPs and childhood-onset asthma in a Mexican population. MATERIALS AND METHODS: In a case-control study 242 unrelated patients with diagnosis of asthma and 358 ethnically- and sex-matched healthy individuals were included. The -617C/A and -653G/ANFE2L2 genotyping was carried out using the TaqMan allelic discrimination assay. RESULTS: The risk allele of both polymorphisms showed a high frequency in our sample (-617A: 24% and -653A: 40%), similarly to those previously reported in Asiatic populations (-617A: 24-29% and -653A: 42-52%; p > 0.05). In contrast, the -617A allele frequency was higher than that reported in a European-African admixed population (10%, p < 0.001). The allelic and genotypic frequencies from both polymorphisms showed no significant differences among cases and controls in female and male samples. Likewise, haplotype analysis found no association between NFE2L2 gene variants and the disease. CONCLUSIONS: Despite the experimental evidence suggesting that NFE2L2 gene is involved in asthma pathogenesis, the -617C/A and -653G/A SNPs were not associated with childhood-onset asthma.
Authors: Venkatesh Sampath; Jeffery S Garland; Daniel Helbling; David Dimmock; Neil P Mulrooney; Pippa M Simpson; Jeffrey C Murray; John M Dagle Journal: Pediatr Res Date: 2014-12-17 Impact factor: 3.756
Authors: Montserrat Rojo de la Vega; Matthew Dodson; Christine Gross; Heidi M Mansour; R Clark Lantz; Eli Chapman; Ting Wang; Stephen M Black; Joe G N Garcia; Donna D Zhang Journal: Curr Pharmacol Rep Date: 2016-02-06
Authors: Elisabet Johansson; Lisa J Martin; Hua He; Xiaoting Chen; Matthew T Weirauch; John W Kroner; Gurjit K Khurana Hershey; Jocelyn M Biagini Journal: Clin Exp Allergy Date: 2021-02-13 Impact factor: 5.401