An investigation of factors affecting the accuracy of in vivo measurements of skin pigments by reflectance spectrophotometry.

Factors affecting the accuracy of the in vivo measurement of cutaneous pigments and blood oxygenation by reflectance spectrophotometry have been examined. It was found that stray light, the amounts of haemoglobin and melanin, and the level of blood oxygenation all contributed to the measured reflectance and had to be taken into account when calculating quantitative indices of skin pigments. Measurements on isolated sheets of epidermis demonstrated that over 50% of normally incident radiation is transmitted in a forward direction within 17 degrees of the incident direction and approximately 20% is backscattered between 90 degrees and 180 degrees out of the sample, approximately 6.0% of it by specular reflection at the surface. The effective optical pathlength in suspensions of whole red cells was found to be 7% greater than in simple solutions containing the same concentration of haemoglobin.