PURPOSE: Tuberculosis (TB) is endemic in India and the burden of multi-drug-resistant tuberculosis (MDR-TB) is high. Early detection of MDR-TB is of primary importance in controlling the spread of TB. The microscopic observational drug susceptibility (MODS) assay has been described as a cost-effective and rapid method by which mycobacterial culture and the drug susceptibility test (DST) can be done at the same time. MATERIALS AND METHODS: A total of 302 consecutive sputum samples that were received in an accredited mycobacteriology laboratory for conventional culture and DST were evaluated by the MODS assay. RESULTS: In comparison with conventional culture on Lowenstein Jensen (LJ) media, the MODS assay showed a sensitivity of 94.12% and a specificity of 89.39% and its concordance with the DST by the proportion method on LJ media to isoniazid and rifampicin was 90.8% and 91.5%, respectively. The turnaround time for results by MODS was 9 days compared to 21 days by culture on LJ media and an additional 42 days for DST by the 1% proportion method. The cost of performing a single MODS assay was Rs. 250/-, compared to Rs. 950/- for culture and 1st line DST on LJ. CONCLUSION: MODS was found to be a sensitive and rapid alternative method for performing culture and DST to identify MDR-TB in resource poor settings.
PURPOSE:Tuberculosis (TB) is endemic in India and the burden of multi-drug-resistant tuberculosis (MDR-TB) is high. Early detection of MDR-TB is of primary importance in controlling the spread of TB. The microscopic observational drug susceptibility (MODS) assay has been described as a cost-effective and rapid method by which mycobacterial culture and the drug susceptibility test (DST) can be done at the same time. MATERIALS AND METHODS: A total of 302 consecutive sputum samples that were received in an accredited mycobacteriology laboratory for conventional culture and DST were evaluated by the MODS assay. RESULTS: In comparison with conventional culture on Lowenstein Jensen (LJ) media, the MODS assay showed a sensitivity of 94.12% and a specificity of 89.39% and its concordance with the DST by the proportion method on LJ media to isoniazid and rifampicin was 90.8% and 91.5%, respectively. The turnaround time for results by MODS was 9 days compared to 21 days by culture on LJ media and an additional 42 days for DST by the 1% proportion method. The cost of performing a single MODS assay was Rs. 250/-, compared to Rs. 950/- for culture and 1st line DST on LJ. CONCLUSION: MODS was found to be a sensitive and rapid alternative method for performing culture and DST to identify MDR-TB in resource poor settings.
Authors: F Rageade; N Picot; A Blanc-Michaud; S Chatellier; C Mirande; E Fortin; A van Belkum Journal: Eur J Clin Microbiol Infect Dis Date: 2014-04-24 Impact factor: 3.267
Authors: M Sanogo; B Kone; B Diarra; M Maiga; B Baya; A M Somboro; Y S Sarro; A C G Togo; B P P Dembele; D Goita; A Kone; O M'Baye; N Coulibaly; S Diabate; B Traore; M H Diallo; Y I Coulibaly; P Saleeb; M Belson; S Orsega; S Siddiqui; M A Polis; S Dao; R L Murphy; S Diallo Journal: Clin Microbiol Infect Date: 2017-01-18 Impact factor: 8.067
Authors: Benson R Kidenya; Rodrick Kabangila; Robert N Peck; Stephen E Mshana; Lauren E Webster; Serena P Koenig; Warren D Johnson; Daniel W Fitzgerald Journal: PLoS One Date: 2013-02-28 Impact factor: 3.240