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Literature DB >> 22359165

Polystyrene substratum for bulk culture of anchorage dependent cells.

Abstract

Twisted ribbons made of polystyrene were used as a packing material for the cultivation of anchorage dependent cells. Normal human fibroblast cells grown on this support in a laboratory scale reactor reached densities of about 5-7×10(5) cells/ml. The cells adhered strongly to the carrier and no cell detachment was observed upon transfer to serum free medium. The properties of this packing material and its potential use are discussed.

Entities: Chemical Species

Year: 1988 PMID： 22359165 DOI： 10.1007/BF00365075

Source DB: PubMed Journal: Cytotechnology ISSN： 0920-9069 Impact factor: 2.058

11 in total

1. The production of foot-and-mouth disease virus from BHK 21 C 13 cells grown on the surface of glass spheres.

Authors: R E Spier; J P Whiteside
Journal: Biotechnol Bioeng Date: 1976-05 Impact factor: 4.530

2. Trypsinization of BHK 21 monolayer cells grown in two large-scale unit process systems.

Authors: R E Spier; J P Whiteside; K Bolt
Journal: Biotechnol Bioeng Date: 1977-11 Impact factor: 4.530

3. The "rotary column" method for growth of large-scale quantities of cell monolayers.

Authors: G G Santero
Journal: Biotechnol Bioeng Date: 1972-09 Impact factor: 4.530

4. Growth of cell-strains and primary cells on micro-carriers in homogeneous culture.

Authors: A L van Wezel
Journal: Nature Date: 1967-10-07 Impact factor: 49.962

5. Method for bulk culture of animal cells on plastic film.

Authors: W House; M Shearer; N G Maroudas
Journal: Exp Cell Res Date: 1972 Impact factor: 3.905

6. Monolayer mass culture on disposable plastic spirals.

Authors: N G Maroudas
Journal: Methods Cell Biol Date: 1974 Impact factor: 1.441

7. Sulphonated polystyrene as an optimal substratum for the adhesion and spreading of mesenchymal cells in monovalent and divalent saline solutions.

Authors: N G Maroudas
Journal: J Cell Physiol Date: 1977-03 Impact factor: 6.384

Polystyrene substratum for bulk culture of anchorage dependent cells.

1. The production of foot-and-mouth disease virus from BHK 21 C 13 cells grown on the surface of glass spheres.

2. Trypsinization of BHK 21 monolayer cells grown in two large-scale unit process systems.

3. The "rotary column" method for growth of large-scale quantities of cell monolayers.

4. Growth of cell-strains and primary cells on micro-carriers in homogeneous culture.

5. Method for bulk culture of animal cells on plastic film.

6. Monolayer mass culture on disposable plastic spirals.

7. Sulphonated polystyrene as an optimal substratum for the adhesion and spreading of mesenchymal cells in monovalent and divalent saline solutions.

8. Visualization of cellular aggregates cultured on a three dimensional collagen sponge matrix.

9. Adhesion of cells to polystyrene surfaces.

10. Stimulation of clonal growth of normal fibroblasts with substrata coated with basic polymers.

1. Fixed bed porous glass sphere (porosphere) bioreactors for animal cells.

2. Test of a novel transparent floating carrier by recombinant cho cells expressing sIL6 receptor protein.

3. Chemically well-defined self-assembled monolayers for cell culture: toward mimicking the natural ECM.