Literature DB >> 22359122

A simple freezing medium for serum-free cultured cells.

T Ohno1, K Kurita, S Abe, N Eimori, Y Ikawa.   

Abstract

Methylcellulose was found to protect serum-free cultured cells from the deleterious effects of freezing and thawing. We have formulated a simple medium suitable for freezing serum-free cultured cells that consists of 0.1% methylcellulose, 10% dimethylsulfoxide, and MEM or any other serum-free culture medium.

Entities:  

Year:  1988        PMID： 22359122     DOI： 10.1007/BF00145029

Source DB:  PubMed          Journal:  Cytotechnology        ISSN： 0920-9069            Impact factor:   2.058


  4 in total

1.  Improved synthetic media suitable for tissue cllture of various mammalian cells.

Authors:  H Katsuta; T Takaoka
Journal:  Methods Cell Biol       Date:  1976       Impact factor: 1.441

2.  Cultivation of strain HeLa cells (cervical carcinoma of human uterus) in a protein-free medium.

Authors:  H KATSUTA; T TAKAOKA; T FURUKAWA; M KAWANA
Journal:  Jpn J Exp Med       Date:  1960-04

3.  Cultivation of strain L cells (mouse fibroblasts) in protein-free media.

Authors:  H KATSUTA; T TAKAOKA; K MITAMURA; I KAWADA; H KUWABARA; S KUWABARA
Journal:  Jpn J Exp Med       Date:  1959-06

4.  Enhanced formation of mouse hybridomas without hat treatment in a serum-free medium.

Authors:  N Yabe; Y Matsuya; I Yamane; M Takada
Journal:  In Vitro Cell Dev Biol       Date:  1986-07
  4 in total
  4 in total

1.  A simple method for in situ freezing of anchorage-dependent cells including rat liver parenchymal cells.

Authors:  T Ohno; K Saijo-Kurita; N Miyamoto-Eimori; T Kurose; Y Aoki; S Yosimura
Journal:  Cytotechnology       Date:  1991-03       Impact factor: 2.058

Review 2.  Cryopreservation of Adipose-Derived Mesenchymal Stem Cells.

Authors:  Chika Miyagi-Shiohira; Kiyoto Kurima; Naoya Kobayashi; Issei Saitoh; Masami Watanabe; Yasufumi Noguchi; Masayuki Matsushita; Hirofumi Noguchi
Journal:  Cell Med       Date:  2015-08-26

3.  Evaluation of methylcellulose and dimethyl sulfoxide as the cryoprotectants in a serum-free freezing media for cryopreservation of adipose-derived adult stem cells.

Authors:  Sreedhar Thirumala; Jeffrey M Gimble; Ram V Devireddy
Journal:  Stem Cells Dev       Date:  2010-04       Impact factor: 3.272

4.  Primary culture and cryopreservation of mouse astrocytes under serum-free conditions.

Authors:  T Yoshida; M Takeuchi
Journal:  Cytotechnology       Date:  1991-02       Impact factor: 2.058
  4 in total

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