The economic production of anti-microbial factor from human promyelocytes in low serum containing medium under chemostat cultivation.

It proves that a purifed Anti-Microbial Factor (AMF) from human promyelocytes has strong activity on Gram(-) and Gram(+) bacteria, showing 0.5 (μg/ml) of Minimal Bacterical Concentration (MBC) on bothE. coli andS. aureus. For mass production of AMF, chemostat cultivation is recommended to accumulate cells out of the reactor since it is an intracellular protein and its system requires only 1% serum in the medium. Its production process proves to be closely growth-related. 1.7×10(-8) (μg/viable cell/day) of maximum specific AMF production rate is estimated at 0.026 h(-1) of dilution rate, maintaining 6×10(6) (viable cell/ml). Ca. 300 (mg/ml) of crude AMF can be obtained for 50 days of continuous cultivation under optimal conditions. The cell growth reaches relatively fast steady state.