Literature DB >> 22358434

Detection of chromosome variation in interphase by in situ hybridization with repetitive DNA probes: potential applications to cytogenetic analysis and mutagenicity testing.

E Raimondi1, S Scariolo, P Vagnarelli, A De Sario, L De Carli.   

Abstract

Individual chromosomes can be identified by means of in situ hybridization with DNA probes for chromosome-specific repetitive sequences. The efficiency and sensitivity of the method are strictly dependent on the characteristics of the probes and the experimental conditions. Using three probes with different copy numbers, we demonstrated that the target chromosomes can be visualized in interphase when the homologous sequences are repeated at least 50 times.Possible applications of interphase analysis to clinical cytogenetics and mutagenicity testing are discussed.

Year:  1987        PMID： 22358434     DOI： 10.1007/BF00351116

Source DB:  PubMed          Journal:  Cytotechnology        ISSN： 0920-9069            Impact factor:   2.058


  8 in total

1.  Detection of chromosome aberrations in the human interphase nucleus by visualization of specific target DNAs with radioactive and non-radioactive in situ hybridization techniques: diagnosis of trisomy 18 with probe L1.84.

Authors:  T Cremer; J Landegent; A Brückner; H P Scholl; M Schardin; H D Hager; P Devilee; P Pearson; M van der Ploeg
Journal:  Hum Genet       Date:  1986-12       Impact factor: 4.132

2.  DNA probe localization at 18p113 band by in situ hybridization and identification of a small supernumerary chromosome.

Authors:  M G Mattei; N Philip; E Passage; J P Moisan; J L Mandel; J F Mattei
Journal:  Hum Genet       Date:  1985       Impact factor: 4.132

3.  Sex chromosome positions in human interphase nuclei as studied by in situ hybridization with chromosome specific DNA probes.

Authors:  G A Rappold; T Cremer; H D Hager; K E Davies; C R Müller; T Yang
Journal:  Hum Genet       Date:  1984       Impact factor: 4.132

4.  Isolation and characterization of an alphoid centromeric repeat family from the human Y chromosome.

Authors:  J Wolfe; S M Darling; R P Erickson; I W Craig; V J Buckle; P W Rigby; H F Willard; P N Goodfellow
Journal:  J Mol Biol       Date:  1985-04-20       Impact factor: 5.469

5.  Localization of single copy DNA sequences of G-banded human chromosomes by in situ hybridization.

Authors:  M E Harper; G F Saunders
Journal:  Chromosoma       Date:  1981       Impact factor: 4.316

6.  Genetic analysis of the fragile-X mental retardation syndrome with two flanking polymorphic DNA markers.

Authors:  I Oberlé; R Heilig; J P Moisan; C Kloepfer; G M Mattéi; J F Mattéi; J Boué; U Froster-Iskenius; P A Jacobs; G M Lathrop
Journal:  Proc Natl Acad Sci U S A       Date:  1986-02       Impact factor: 11.205

7.  The organization of two related subfamilies of a human tandemly repeated DNA is chromosome specific.

Authors:  M Jeanpierre; D Weil; P Gallano; N Creau-Goldberg; C Junien
Journal:  Hum Genet       Date:  1985       Impact factor: 4.132

8.  Cloning of a representative genomic library of the human X chromosome after sorting by flow cytometry.

Authors:  K E Davies; B D Young; R G Elles; M E Hill; R Williamson
Journal:  Nature       Date:  1981-10-01       Impact factor: 49.962
  8 in total

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