Literature DB >> 22350885

Isolation of ubiquitylated proteins using tandem ubiquitin-binding entities.

Fabienne Aillet1, Fernando Lopitz-Otsoa, Roland Hjerpe, Mónica Torres-Ramos, Valérie Lang, Manuel S Rodríguez.   

Abstract

Studying postubiquitylation events has always been a difficult task due to the labile nature of these posttranslational modifications. When utilized in tandem, ubiquitin-binding entities (TUBEs) not only increase up to thousand times the affinity for poly-ubiquitin chains but also protect ubiquitylated proteins from the action of the proteasome and de-ubiquitylating enzymes.

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Year:  2012        PMID： 22350885     DOI： 10.1007/978-1-61779-474-2_12

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  16 in total

1.  Analysis of defective protein ubiquitylation associated to adriamycin resistant cells.

Authors:  Valérie Lang; Fabienne Aillet; Wendy Xolalpa; Sonia Serna; Laurie Ceccato; Rosa G Lopez-Reyes; Maria Paz Lopez-Mato; Radosław Januchowski; Niels-Christian Reichardt; Manuel S Rodriguez
Journal:  Cell Cycle       Date:  2017-11-20       Impact factor: 4.534

Review 2.  Enzyme-substrate relationships in the ubiquitin system: approaches for identifying substrates of ubiquitin ligases.

Authors:  Hazel F O'Connor; Jon M Huibregtse
Journal:  Cell Mol Life Sci       Date:  2017-04-28       Impact factor: 9.261

3.  Exploring selective autophagy events in multiple biologic models using LC3-interacting regions (LIR)-based molecular traps.

Authors:  Grégoire Quinet; Pierre Génin; Oznur Ozturk; Naima Belgareh-Touzé; Lilas Courtot; Renaud Legouis; Robert Weil; Mickael M Cohen; Manuel S Rodriguez
Journal:  Sci Rep       Date:  2022-05-10       Impact factor: 4.996

4.  Acute unfolding of a single protein immediately stimulates recruitment of ubiquitin protein ligase E3C (UBE3C) to 26S proteasomes.

Authors:  Colin D Gottlieb; Airlia C S Thompson; Alban Ordureau; J Wade Harper; Ron R Kopito
Journal:  J Biol Chem       Date:  2019-08-02       Impact factor: 5.157

5.  Central angiotensin II-Protein inhibitor of neuronal nitric oxide synthase (PIN) axis contribute to neurogenic hypertension.

Authors:  Neeru M Sharma; Andrea S Haibara; Kenichi Katsurada; Xuefei Liu; Kaushik P Patel
Journal:  Nitric Oxide       Date:  2019-10-22       Impact factor: 4.427

6.  Mdm2 regulates cardiac contractility by inhibiting GRK2-mediated desensitization of β-adrenergic receptor signaling.

Authors:  Pierre-Yves Jean-Charles; Samuel Mon-Wei Yu; Dennis Abraham; Reddy Peera Kommaddi; Lan Mao; Ryan T Strachan; Zhu-Shan Zhang; Dawn E Bowles; Leigh Brian; Jonathan A Stiber; Stephen N Jones; Walter J Koch; Howard A Rockman; Sudha K Shenoy
Journal:  JCI Insight       Date:  2017-09-07

7.  Tetramerization-defects of p53 result in aberrant ubiquitylation and transcriptional activity.

Authors:  Valérie Lang; Chiara Pallara; Amaia Zabala; Sofia Lobato-Gil; Fernando Lopitz-Otsoa; Rosa Farrás; Roland Hjerpe; Monica Torres-Ramos; Lorea Zabaleta; Christine Blattner; Ronald T Hay; Rosa Barrio; Arkaitz Carracedo; Juan Fernandez-Recio; Manuel S Rodríguez; Fabienne Aillet
Journal:  Mol Oncol       Date:  2014-04-13       Impact factor: 6.603

8.  BLAP-Tags, TUBEs and DUB-Chips: Combined Novel Technologies will Advance Molecular Epithelial Physiology.

Authors:  Kirk L Hamilton
Journal:  Front Physiol       Date:  2012-05-15       Impact factor: 4.566

9.  Heterologous SUMO-2/3-ubiquitin chains optimize IκBα degradation and NF-κB activity.

Authors:  Fabienne Aillet; Fernando Lopitz-Otsoa; Isabel Egaña; Roland Hjerpe; Paul Fraser; Ron T Hay; Manuel S Rodriguez; Valérie Lang
Journal:  PLoS One       Date:  2012-12-20       Impact factor: 3.240

Review 10.  Ubiquitin chain topology in plant cell signaling: a new facet to an evergreen story.

Authors:  Charlotte K Walsh; Ari Sadanandom
Journal:  Front Plant Sci       Date:  2014-04-01       Impact factor: 5.753
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