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Literature DB >> 22344638

Lytic activity of LysH5 endolysin secreted by Lactococcus lactis using the secretion signal sequence of bacteriocin Lcn972.

Lorena Rodríguez-Rubio¹, Dolores Gutiérrez, Beatriz Martínez, Ana Rodríguez, Pilar García.

Abstract

Bacteriophage endolysins have an interesting potential as antimicrobials. The endolysin LysH5, encoded by Staphylococcus aureus phage vB_SauS-phi-IPLA88, was expressed and secreted in Lactococcus lactis using the signal peptide of bacteriocin lactococcin 972 and lactococcal constitutive and inducible promoters. Up to 80 U/mg of extracellular active endolysin was detected in culture supernatants, but most of the protein (up to 323 U/mg) remained in the cell extracts.

Entities: Chemical Species

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Year: 2012 PMID： 22344638 PMCID： PMC3346474 DOI： 10.1128/AEM.00018-12

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

36 in total

1. Lytic activity of recombinant bacteriophage phi11 and phi12 endolysins on whole cells and biofilms of Staphylococcus aureus.

Authors: Peter Sass; Gabriele Bierbaum
Journal: Appl Environ Microbiol Date: 2006-11-03 Impact factor: 4.792

2. High-level heterologous production and functional expression of the sec-dependent enterocin P from Enterococcus faecium P13 in Lactococcus lactis.

Authors: Jorge Gutiérrez; Rasmus Larsen; Luis M Cintas; Jan Kok; Pablo E Hernández
Journal: Appl Microbiol Biotechnol Date: 2006-01-17 Impact factor: 4.813

Review 3. Implication of milk and milk products in food-borne diseases in France and in different industrialised countries.

Authors: M L De Buyser; B Dufour; M Maire; V Lafarge
Journal: Int J Food Microbiol Date: 2001-07-20 Impact factor: 5.277

4. Use of the usp45 lactococcal secretion signal sequence to drive the secretion and functional expression of enterococcal bacteriocins in Lactococcus lactis.

Authors: Juan Borrero; Juan J Jiménez; Loreto Gútiez; Carmen Herranz; Luis M Cintas; Pablo E Hernández
Journal: Appl Microbiol Biotechnol Date: 2010-09-15 Impact factor: 4.813

5. Heterologous coproduction of enterocin A and pediocin PA-1 by Lactococcus lactis: detection by specific peptide-directed antibodies.

Authors: J M Martínez; J Kok; J W Sanders; P E Hernández
Journal: Appl Environ Microbiol Date: 2000-08 Impact factor: 4.792

6. Construction of a vector plasmid family and its use for molecular cloning in Streptococcus lactis.

Authors: D Simon; A Chopin
Journal: Biochimie Date: 1988-04 Impact factor: 4.079

7. Functional genomic analysis of two Staphylococcus aureus phages isolated from the dairy environment.

Authors: Pilar García; Beatriz Martínez; José María Obeso; Rob Lavigne; Rudi Lurz; Ana Rodríguez
Journal: Appl Environ Microbiol Date: 2009-10-16 Impact factor: 4.792

Review 8. Lactococcus lactis, an efficient cell factory for recombinant protein production and secretion.

Authors: E Morello; L G Bermúdez-Humarán; D Llull; V Solé; N Miraglio; P Langella; I Poquet
Journal: J Mol Microbiol Biotechnol Date: 2008

9. Receptor binding domain of Escherichia coli F18 fimbrial adhesin FedF can be both efficiently secreted and surface displayed in a functional form in Lactococcus lactis.

Authors: Agneta Lindholm; Andreas Smeds; Airi Palva
Journal: Appl Environ Microbiol Date: 2004-04 Impact factor: 4.792

10. Invasive potential of biofilm-forming Staphylococci bovine subclinical mastitis isolates.

Authors: Manuela Oliveira; Ricardo Bexiga; Sandro Filipe Nunes; Cristina Lobo Vilela
Journal: J Vet Sci Date: 2011-03 Impact factor: 1.672

11 in total

1. Antimicrobial peptides targeting Gram-negative pathogens, produced and delivered by lactic acid bacteria.

Authors: Katherine Volzing; Juan Borrero; Michael J Sadowsky; Yiannis N Kaznessis
Journal: ACS Synth Biol Date: 2013-07-10 Impact factor: 5.110

Review 2. Recombinant Endolysins as Potential Therapeutics against Antibiotic-Resistant Staphylococcus aureus: Current Status of Research and Novel Delivery Strategies.

Authors: Hamed Haddad Kashani; Mathias Schmelcher; Hamed Sabzalipoor; Elahe Seyed Hosseini; Rezvan Moniri
Journal: Clin Microbiol Rev Date: 2017-11-29 Impact factor: 26.132

Lytic activity of LysH5 endolysin secreted by Lactococcus lactis using the secretion signal sequence of bacteriocin Lcn972.

1. Lytic activity of recombinant bacteriophage phi11 and phi12 endolysins on whole cells and biofilms of Staphylococcus aureus.

2. High-level heterologous production and functional expression of the sec-dependent enterocin P from Enterococcus faecium P13 in Lactococcus lactis.

Review 3. Implication of milk and milk products in food-borne diseases in France and in different industrialised countries.

4. Use of the usp45 lactococcal secretion signal sequence to drive the secretion and functional expression of enterococcal bacteriocins in Lactococcus lactis.

5. Heterologous coproduction of enterocin A and pediocin PA-1 by Lactococcus lactis: detection by specific peptide-directed antibodies.

6. Construction of a vector plasmid family and its use for molecular cloning in Streptococcus lactis.

7. Functional genomic analysis of two Staphylococcus aureus phages isolated from the dairy environment.

Review 8. Lactococcus lactis, an efficient cell factory for recombinant protein production and secretion.

9. Receptor binding domain of Escherichia coli F18 fimbrial adhesin FedF can be both efficiently secreted and surface displayed in a functional form in Lactococcus lactis.

10. Invasive potential of biofilm-forming Staphylococci bovine subclinical mastitis isolates.

1. Antimicrobial peptides targeting Gram-negative pathogens, produced and delivered by lactic acid bacteria.

Review 2. Recombinant Endolysins as Potential Therapeutics against Antibiotic-Resistant Staphylococcus aureus: Current Status of Research and Novel Delivery Strategies.

Review 3. Role of Bacteriophages in the Implementation of a Sustainable Dairy Chain.

Review 4. Peptidoglycan hydrolases-potential weapons against Staphylococcus aureus.

5. Expression and lytic efficacy assessment of the Staphylococcus aureus phage SA4 lysin gene.

6. Mitrecin A, an endolysin-like bacteriolytic enzyme from a newly isolated soil streptomycete.

Review 7. Bacteriophages as Weapons Against Bacterial Biofilms in the Food Industry.

Review 8. Biocontrol and Rapid Detection of Food-Borne Pathogens Using Bacteriophages and Endolysins.

Review 9. Bacteriophage-Derived Endolysins Applied as Potent Biocontrol Agents to Enhance Food Safety.

10. Downstream Processing of Chlamydomonas reinhardtii TN72 for Recombinant Protein Recovery.