OBJECTIVE: The objective of the study was to determine whether the bacterial load of genital mycoplasmas and gestation age are related to intraamniotic inflammatory response using the amniotic fluid levels of 18 inflammatory mediators. STUDY DESIGN: A prospective study of 145 women with singleton pregnancies complicated by preterm prelabor rupture of membranes between 24(0/7) and 36(6/7) weeks was conducted. Amniotic fluid was obtained from all women by transabdominal amniocentesis. The amounts of genital mycoplasma deoxyribonucleic acid were determined using the threshold cycle value and relative and absolute quantification techniques. A panel of multiple proteins was analyzed simultaneously using multiplex technology. RESULTS: Twenty-four women with the presence of genital mycoplasmas in amniotic fluid were included in the final analyses. The concentrations of 9 of the 18 evaluated proteins in the amniotic fluid correlated with bacterial load of genital mycoplasmas independent of the quantification technique used. CONCLUSION: The intensity of intraamniotic inflammatory response to genital mycoplasmas decreased with gestational age.
OBJECTIVE: The objective of the study was to determine whether the bacterial load of genital mycoplasmas and gestation age are related to intraamniotic inflammatory response using the amniotic fluid levels of 18 inflammatory mediators. STUDY DESIGN: A prospective study of 145 women with singleton pregnancies complicated by preterm prelabor rupture of membranes between 24(0/7) and 36(6/7) weeks was conducted. Amniotic fluid was obtained from all women by transabdominal amniocentesis. The amounts of genital mycoplasma deoxyribonucleic acid were determined using the threshold cycle value and relative and absolute quantification techniques. A panel of multiple proteins was analyzed simultaneously using multiplex technology. RESULTS: Twenty-four women with the presence of genital mycoplasmas in amniotic fluid were included in the final analyses. The concentrations of 9 of the 18 evaluated proteins in the amniotic fluid correlated with bacterial load of genital mycoplasmas independent of the quantification technique used. CONCLUSION: The intensity of intraamniotic inflammatory response to genital mycoplasmas decreased with gestational age.
Authors: Ivana Musilova; Ctirad Andrys; Marcela Drahosova; Barbora Zednikova; Helena Hornychova; Lenka Pliskova; Helena Zemlickova; Bo Jacobsson; Marian Kacerovsky Journal: Pediatr Res Date: 2017-12-20 Impact factor: 3.756
Authors: Peta L Grigsby; Miles J Novy; Drew W Sadowsky; Terry K Morgan; Mary Long; Ed Acosta; Lynn B Duffy; Ken B Waites Journal: Am J Obstet Gynecol Date: 2012-10-23 Impact factor: 8.661
Authors: Marian Kacerovsky; Roberto Romero; Lenka Pliskova; Radka Bolehovska; Helena Hornychova; Adela Matejkova; Hana Vosmikova; Ctirad Andrys; Martina Kolackova; Piotr Laudański; Vera Pelantova; Bo Jacobsson; Ivana Musilova Journal: J Matern Fetal Neonatal Med Date: 2019-07-15
Authors: Jeffrey A Keelan; Matthew W Kemp; Matthew S Payne; David Johnson; Sarah J Stock; Masatoshi Saito; Prabhavathi Fernandes; John P Newnham Journal: Antimicrob Agents Chemother Date: 2013-11-04 Impact factor: 5.191
Authors: Teresa Cobo; Marian Kacerovsky; Montse Palacio; Helena Hornychova; David M Hougaard; Kristin Skogstrand; Bo Jacobsson Journal: PLoS One Date: 2012-08-20 Impact factor: 3.240