AIM: To investigate the mechanism(s) by which the intestinal commensal microbe Lactobacillus acidophilus can affect host immunity, we studied the role of a component of the cell wall, lipoteichoic acid, in colitis. MATERIALS & METHODS: Colitis was induced by the intraperitoneal injection of pathogenic CD4(+)CD25(-)CD45RB(hi) T cells into Rag1(-/-) mice. The parental strain, NCK56, or the lipoteichoic acid-deficient strain, NCK2025, was then administered orally. Fluorescent microscopy was employed to examine resulting cell populations and their cytokine production in the colon. RESULTS: NCK2025 enhanced IL-10 production by dendritic cells and macrophages. Increased numbers of regulatory dendritic cells coincided with the induction of activated FoxP3(+) Tregs. CONCLUSION: These results suggest that the oral administration of the genetically modified strain NCK2025 may be an effective immunotherapeutic approach that reprograms the immune response in colonic inflammatory conditions.
AIM: To investigate the mechanism(s) by which the intestinal commensal microbe Lactobacillus acidophilus can affect host immunity, we studied the role of a component of the cell wall, lipoteichoic acid, in colitis. MATERIALS & METHODS:Colitis was induced by the intraperitoneal injection of pathogenic CD4(+)CD25(-)CD45RB(hi) T cells into Rag1(-/-) mice. The parental strain, NCK56, or the lipoteichoic acid-deficient strain, NCK2025, was then administered orally. Fluorescent microscopy was employed to examine resulting cell populations and their cytokine production in the colon. RESULTS: NCK2025 enhanced IL-10 production by dendritic cells and macrophages. Increased numbers of regulatory dendritic cells coincided with the induction of activated FoxP3(+) Tregs. CONCLUSION: These results suggest that the oral administration of the genetically modified strain NCK2025 may be an effective immunotherapeutic approach that reprograms the immune response in colonic inflammatory conditions.
Authors: Mansour Mohamadzadeh; Erika A Pfeiler; Jeffrey B Brown; Mojgan Zadeh; Matthew Gramarossa; Elizabeth Managlia; Praveen Bere; Bara Sarraj; Mohammad W Khan; Krishna Chaitanya Pakanati; M Javeed Ansari; Sarah O'Flaherty; Terrence Barrett; Todd R Klaenhammer Journal: Proc Natl Acad Sci U S A Date: 2011-01-31 Impact factor: 11.205
Authors: Charles O Elson; Yingzi Cong; Vance J McCracken; Reed A Dimmitt; Robin G Lorenz; Casey T Weaver Journal: Immunol Rev Date: 2005-08 Impact factor: 12.988
Authors: Daniela M Remus; Roger S Bongers; Marjolein Meijerink; Fabrizia Fusetti; Bert Poolman; Paul de Vos; Jerry M Wells; Michiel Kleerebezem; Peter A Bron Journal: J Bacteriol Date: 2012-11-21 Impact factor: 3.490