Application of reverse-DEPT polarization transfer pulse sequence to study the metabolism of carbon-13-labeled substrates in perfused organs by 1H NMR spectroscopy.

Metabolism of 13C-enriched metabolites can be advantageously studied by reverse-polarization transfer methods. In this work an improved reverse-DEPT sequence has been applied for the first time on perfused organs in a 20-mm probe. The metabolic fate of 99% enriched [2-13C]acetate perfused in excised rat liver and heart has been documented.