OBJECTIVE: The adventitia is increasingly recognized as an important player during the development of intimal hyperplasia. However, the mechanism of adventitial cell recruitment to the subintimal space remains largely undefined. We have shown previously that gene transfer of protein kinase C-delta (PKCδ) increases apoptosis of smooth muscle cells following balloon injury. In the current study, we investigated a potential role of PKCδ in regulating the recruitment of adventitial cells. METHODS AND RESULTS: Conditioned media from PKCδ-overexpressing smooth muscle cells stimulated migration and CCR2 expression of adventitial fibroblasts through a MCP-1 dependent mechanism. Following balloon injury of rat carotid arteries, overexpression of PKCδ in smooth muscle cells significantly increased MCP-1 and CCR2 expression and the number of adventitia-originated cells detected in the neointima. Administration of an anti-MCP-1 antibody markedly diminished the recruitment of adventitial cells. Combined PKCδ overexpression and anti-MCP-1 inhibited intimal hyperplasia more effectively than either approach alone. CONCLUSIONS: Our data suggest that PKCδ regulates recruitment of adventitial cells to the neointima via a mechanism involving upregulation of the MCP-1/CCR2 signaling axis in injured arteries. Blockage of MCP-1 while enhancing apoptosis may serve as a potential therapeutic strategy to attenuate intimal hyperplasia.
OBJECTIVE: The adventitia is increasingly recognized as an important player during the development of intimal hyperplasia. However, the mechanism of adventitial cell recruitment to the subintimal space remains largely undefined. We have shown previously that gene transfer of protein kinase C-delta (PKCδ) increases apoptosis of smooth muscle cells following balloon injury. In the current study, we investigated a potential role of PKCδ in regulating the recruitment of adventitial cells. METHODS AND RESULTS: Conditioned media from PKCδ-overexpressing smooth muscle cells stimulated migration and CCR2 expression of adventitial fibroblasts through a MCP-1 dependent mechanism. Following balloon injury of rat carotid arteries, overexpression of PKCδ in smooth muscle cells significantly increased MCP-1 and CCR2 expression and the number of adventitia-originated cells detected in the neointima. Administration of an anti-MCP-1 antibody markedly diminished the recruitment of adventitial cells. Combined PKCδ overexpression and anti-MCP-1 inhibited intimal hyperplasia more effectively than either approach alone. CONCLUSIONS: Our data suggest that PKCδ regulates recruitment of adventitial cells to the neointima via a mechanism involving upregulation of the MCP-1/CCR2 signaling axis in injured arteries. Blockage of MCP-1 while enhancing apoptosis may serve as a potential therapeutic strategy to attenuate intimal hyperplasia.
Authors: Bethany B Moore; Jill E Kolodsick; Victor J Thannickal; Kenneth Cooke; Thomas A Moore; Cory Hogaboam; Carol A Wilke; Galen B Toews Journal: Am J Pathol Date: 2005-03 Impact factor: 4.307
Authors: Merce Roque; William J H Kim; Michaela Gazdoin; Alia Malik; Ernane D Reis; John T Fallon; Juan J Badimon; Israel F Charo; Mark B Taubman Journal: Arterioscler Thromb Vasc Biol Date: 2002-04-01 Impact factor: 8.311
Authors: William J H Kim; Igor Chereshnev; Mihaela Gazdoiu; John T Fallon; Barrett J Rollins; Mark B Taubman Journal: Biochem Biophys Res Commun Date: 2003-10-24 Impact factor: 3.575
Authors: Daniel M DiRenzo; Mirnal A Chaudhary; Xudong Shi; Sarah R Franco; Joshua Zent; Katie Wang; Lian-Wang Guo; K Craig Kent Journal: Cell Signal Date: 2016-02-19 Impact factor: 4.315