| Literature DB >> 2232864 |
A Alvarez-Buylla1, C Y Ling, J R Kirn.
Abstract
Cresyl violet is widely used by neurobiologists to visualize Nissl substance in bright-field microscopy. Here we describe a method for using this dye as a red fluorescent Nissl stain. Unlike the bright-field staining technique, fluorescent cresyl is compatible with other fluorescent dyes and tracers, such as fluorescein, Fluoro-Gold and Fast Blue. The procedure requires only minor modifications of routine bright-field cresyl staining, the most significant being dilution of the stain. Thus, fluorescent red cresyl violet is simple to implement and may be of general use in fluorescence microscopy.Entities:
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Year: 1990 PMID： 2232864 DOI： 10.1016/0165-0270(90)90016-9
Source DB: PubMed Journal: J Neurosci Methods ISSN： 0165-0270 Impact factor: 2.390