| Literature DB >> 22328378 |
Vitor Cabral1, Murielle Chauvel, Arnaud Firon, Mélanie Legrand, Audrey Nesseir, Sophie Bachellier-Bassi, Yogesh Chaudhari, Carol A Munro, Christophe d'Enfert.
Abstract
Over-expression is a valid functional genomics approach to characterise genes of unknown function on a genome-wide scale. Strains are engineered to over-express a specific gene and the resulting gain-of-function phenotype assessed. Here, we describe the strategy we are adopting to synthesise a Candida albicans ORFeome collection and the options available to create over-expressing strains from this collection.Entities:
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Year: 2012 PMID： 22328378 DOI： 10.1007/978-1-61779-539-8_15
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745