Literature DB >> 22326551

Evaluation of systemic microvascular endothelial function using laser speckle contrast imaging.

Ivan Cordovil1, Grazielle Huguenin, Glorimar Rosa, Annie Bello, Onilmar Köhler, Roger de Moraes, Eduardo Tibiriçá.   

Abstract

OBJECTIVE: The aim of this study was to compare cutaneous microvascular function in young healthy subjects (n=50) with that of cardiometabolic diseased patients (n=50) using laser speckle contrast imaging (LSCI) coupled with transdermal iontophoretic delivery of acetylcholine (ACh) and post-occlusive reactive hyperemia (PORH).
METHODS: Cutaneous blood flow was assessed in the forearm using LSCI at rest, during PORH and during iontophoresis of ACh with increasing anodal currents of 30, 60, 90, 120, 150 and 180 μA during 10-second intervals spaced 1 min apart.
RESULTS: Endothelium-dependent skin microvascular vasodilator responses induced by both ACh and PORH were significantly reduced in cardiometabolic diseased patients compared to healthy subjects. Vasodilator responses induced by ACh were significantly higher in young women than in young men. Iontophoresis charges up to 1.5 mC do not induce nonspecific effects on skin microvascular flux.
CONCLUSION: LSCI appears to be a promising noninvasive technique for evaluating systemic microvascular endothelial function.
Copyright © 2012 Elsevier Inc. All rights reserved.

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Year:  2012        PMID: 22326551     DOI: 10.1016/j.mvr.2012.01.004

Source DB:  PubMed          Journal:  Microvasc Res        ISSN: 0026-2862            Impact factor:   3.514


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