| Literature DB >> 22326416 |
Sylvain Clède1, François Lambert, Christophe Sandt, Zoher Gueroui, Nicolas Delsuc, Paul Dumas, Anne Vessières, Clotilde Policar.
Abstract
1,1-Di(4-hydroxyphenyl)-2-cyrhetrenylbut-1-ene 1 is an organometallic conjugate where a [(Cp)Re(CO)(3)] unit is linked to a hydroxytamoxifen-like structure. Its subcellular nuclear distribution was previously observed in a single cell using the near-field technique AFMIR. We show here that synchrotron radiation FTIR spectromicroscopy (SR-FTIR-SM) enabled the mapping of 1 based on its IR-signature (characteristic bands in the 1850-2200 cm(-1) range) and pointed out the colocalization of 1 with an area of high amide density. Fluorescence microscopy using DAPI staining performed on the same cells confirmed that this area corresponds to the cell nucleus.Entities:
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Year: 2012 PMID： 22326416 DOI： 10.1016/j.biotechadv.2012.01.023
Source DB: PubMed Journal: Biotechnol Adv ISSN： 0734-9750 Impact factor: 14.227