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Literature DB >> 22322354

Contribution of amoebic coculture to recovery of Legionella isolates from respiratory samples: prospective analysis over a period of 32 months.

G Descours¹, A Suet, C Ginevra, C Campese, S Slimani, F Ader, D Che, G Lina, S Jarraud.

Abstract

We evaluated the contribution of amoebic coculture to the recovery of Legionella spp. from 379 respiratory samples. The sensitivity of axenic culture was 42.1%. The combination of axenic culture with amoebic coculture increased the Legionella isolation rate to 47.1%. Amoebic coculture was particularly efficient in isolating Legionella spp. from respiratory samples contaminated with oropharyngeal flora.

Entities: Gene

Mesh：

Year: 2012 PMID： 22322354 PMCID： PMC3347135 DOI： 10.1128/JCM.06531-11

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

12 in total

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Authors: Jürgen H Helbig; Søren A Uldum; Sverker Bernander; Paul Christian Lück; Günther Wewalka; Bill Abraham; Valeria Gaia; Timothy G Harrison
Journal: J Clin Microbiol Date: 2003-02 Impact factor: 5.948

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Authors: Barry S Fields; Robert F Benson; Richard E Besser
Journal: Clin Microbiol Rev Date: 2002-07 Impact factor: 26.132

9. Effective proliferation of low level Legionella pneumophila serogroup 1 cells using coculture procedure with Acanthamoeba castellanii.

Authors: Masato Seno; Miyoko Sakaki; Hiromi Ogawa; Hanako Matsuda; Yoshihiro Takeda
Journal: J Microbiol Methods Date: 2006-03-03 Impact factor: 2.363

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Authors: T J Rowbotham
Journal: J Clin Pathol Date: 1983-09 Impact factor: 3.411

4 in total