| Literature DB >> 22315082 |
P Patrick Dotson1, Jonathan Hart, Christopher Noe, Stephen M Testa.
Abstract
The trans insertion-splicing (TIS) reaction is a technique that can be used to site-specifically insert an RNA donor substrate into a separate RNA acceptor substrate. The TIS reaction, which is catalyzed by a group I intron-derived ribozyme from Pneumocystis carinii, is described with regards to system design, ribozyme preparation, and the overall protocol for conducting the TIS reaction.Entities:
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Year: 2012 PMID： 22315082 DOI： 10.1007/978-1-61779-545-9_24
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745