OBJECT: This study aims to investigate the impact of anti-CD25 monoclonal antibody (basiliximab) on peripheral blood regulatory T cells (Treg) and their subsets in recipients early after renal transplantation. METHODS: Ten renal transplant recipients from November 2009 to February 2011 were divided into an induction therapy and a no-induction therapy group, mainly based on their will to accept basiliximab induction. Peripheral blood samples were collected at 2 hours before as well as 1 and 2 weeks after transplantation. Flow cytometry was used to analyze the proportion of regulatory T cells and their subsets. RESULTS: Compared with the no-induction therapy group, the proportions of both CD25(high) T cell and CD25(high) Foxp3(+) double marked T cells were significantly decreased among in the basiliximab induction group remaining at low at 2 weeks. The subsets of activated Treg and cytokine-secreting Treg were also temporarily downregulated at 1 week after transplantation using basiliximab induction. However, the inhibitory transcriptional factor Foxp3 was not significantly affected by the induction therapy. CONCLUSIONS: Anti-CD25 monoclonal antibody downregulated the proportion of regulatory T cell and its activated subsets in peripheral blood in the early stage after renal transplantation while the inhibitory function may still be spared.
OBJECT: This study aims to investigate the impact of anti-CD25 monoclonal antibody (basiliximab) on peripheral blood regulatory T cells (Treg) and their subsets in recipients early after renal transplantation. METHODS: Ten renal transplant recipients from November 2009 to February 2011 were divided into an induction therapy and a no-induction therapy group, mainly based on their will to accept basiliximab induction. Peripheral blood samples were collected at 2 hours before as well as 1 and 2 weeks after transplantation. Flow cytometry was used to analyze the proportion of regulatory T cells and their subsets. RESULTS: Compared with the no-induction therapy group, the proportions of both CD25(high) T cell and CD25(high) Foxp3(+) double marked T cells were significantly decreased among in the basiliximab induction group remaining at low at 2 weeks. The subsets of activated Treg and cytokine-secreting Treg were also temporarily downregulated at 1 week after transplantation using basiliximab induction. However, the inhibitory transcriptional factor Foxp3 was not significantly affected by the induction therapy. CONCLUSIONS: Anti-CD25 monoclonal antibody downregulated the proportion of regulatory T cell and its activated subsets in peripheral blood in the early stage after renal transplantation while the inhibitory function may still be spared.
Authors: Jose F Camargo; Suresh Pallikkuth; Ilona Moroz; Yoichiro Natori; Maria L Alcaide; Allan Rodriguez; Giselle Guerra; George W Burke; Savita Pahwa Journal: Kidney Int Rep Date: 2019-08-19
Authors: Sybille Landwehr-Kenzel; Anne Zobel; Isabela Schmitt-Knosalla; Anne Forke; Henrike Hoffmann; Michael Schmueck-Henneresse; Robert Klopfleisch; Hans-Dieter Volk; Petra Reinke Journal: Front Immunol Date: 2021-10-11 Impact factor: 7.561
Authors: Mark E Snyder; Michael O Finlayson; Thomas J Connors; Pranay Dogra; Takashi Senda; Erin Bush; Dustin Carpenter; Charles Marboe; Luke Benvenuto; Lori Shah; Hilary Robbins; Jaime L Hook; Megan Sykes; Frank D'Ovidio; Matthew Bacchetta; Joshua R Sonett; David J Lederer; Selim Arcasoy; Peter A Sims; Donna L Farber Journal: Sci Immunol Date: 2019-03-08